Phosphorus tribromide

Endpoint summary

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus tribromide. The study was performed using Salmonella typhimurium frame-shift or base-pair substitution tester strains in both buffered and unbuffered solutions.

 

Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.

Link to relevant study records

Reference

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from HSDB

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus tribromide

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Specific details on test material used for the study:

- Name of test material: Phosphorus tribromide
- IUPAC name: Phosphorus (3+) tribromide
- Molecular formula: Br3P
- Molecular weight: 270.686 g/mol
- Substance type: Inorganic
- Physical state: Colourless liquid
- Purity: No data
- Impurities (identity and concentrations): No data

Target gene:

Histidine

Species / strain / cell type:

S. typhimurium, other: frame shift or base-pair substitution tester strains

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

not specified

Metabolic activation system:

No data

Test concentrations with justification for top dose:

No data

Vehicle / solvent:

No data

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar

DURATION
- Preincubation period: No data
- Exposure duration: No data
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: No data

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data

OTHER: No data

Rationale for test conditions:

No data

Evaluation criteria:

The plates were observed for a dose dependent increase in the number of revertnats/plate

Statistics:

No data

Species / strain:

S. typhimurium, other: frame shift or base-pair substitution tester strains

Metabolic activation:

not specified

Genotoxicity:

negative

Remarks:

in both buffered and unbuffered solutions

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data

Conclusions:

Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.

Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus tribromide. The study was performed using Salmonella typhimurium frame-shift or base-pair substitution tester strains in both buffered and unbuffered solutions. No mutagenic activity was noted in the mentioned strains due to the test chemical.

 

Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (negative)

Genetic toxicity in vivo

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Gene mutation in vitro:

Data available for the target chemical was reviewed to determine the mutagenic nature of phosphorus tribromide (IUPAC name: phosphorus (3+) tribromide). The studies are as mentioned below:

Gene mutation toxicity study was performed (U. S. National Library of Medicine, 2017) to determine the mutagenic nature of Phosphorus tribromide. The study was performed using Salmonella typhimurium frame-shift or base-pair substitution tester strains in both buffered and unbuffered solutions. No mutagenic activity was noted in the mentioned strains due to the test chemical. Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.

In a study for structurally and functionally similar read across chemical by McMohan et al (Cancer Research, 1979), gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus trichloride (RA CAS no 7719 -12 -2, IUPAC name: Phosphorus (3 +) trichloride). The study was performed using Salmonella typhimurium G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98 and E. coli WP2 and WP2 uvrA- with and without Liver enzymes activation system. Ten ml of minimal agar medium (not containing test compound) was poured into a square Petri dish (9 x 9 cm) which is tilted at a slight angle. The agar was then allowed to solidify into a wedge-shaped layer by standing at room temperature. Meanwhile, a 1000-µg/ml mixture of test compound in agar was prepared by adding 10 ml of minimal agar to 0.1 ml of a 100-mg/mI solution of test compound in dimethyl sulfoxide. When appropriate, water or dimethoxyethane was used instead of dimethyl sulfoxide. The cooled agar plates were then placed on a level surface, and an overlay of the 10 ml of agar containing the test compound was poured onto the plate to form a reversed wedge of agar on top of the first wedge. A concentration gradient of compound was produced by allowing the compound in the upper wedge to diffuse into the lower layer for 2 hr at room temperature. The concentration range in this plate is approximately 100 to 1000µg/ml. Three additional plates with concentration ranges of 10 to 100µg/ml, 1 to 10µg/ml, and 0.1 to 1µg/ml were prepared. A streaking device consisting of 10 sterile 50-µL pipets was dipped into suspensions of the 10 test strains and allowed to fill by capillary action. The pipets were then touched to the upper edge of the gradient and drawn across the plate. The study was performed in the presence and absence of liver enzyle activating system and the plates were incubated for 48 hrs at 37°C. Phosphorus trichloride did not induce gene mutation in Salmonella typhimuriumG46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98 and E. coli WP2 and WP2 uvrA- in the presence and absence of Liver enzymes activation systemand hence the chemical is not likely to classify as a gene mutant in vitro.

Based on the information available for the target chemical and its read across, Phosphorus tribromide does not exhibit gene mutation in vitro. Hence the chemical is not likely to classify as a gene mutant in vitro.

Justification for classification or non-classification

Based on the information available for the target chemical and its read across, Phosphorus tribromide does not exhibit gene mutation in vitro. Hence the chemical is not likely to classify as a gene mutant in vitro.