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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Toxicological information

Specific investigations: other studies

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Administrative data

specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable data.

Data source

Reference Type:
Subcellular localization of 14C-glutaraldehyde in liver cells.
Amstutz L, Ranly D
Bibliographic source:
J. Dent. Res. 68: 203, Abstract 176

Materials and methods

Principles of method if other than guideline:
The aim of the study was to determine if glutaraldehyde can associate with the nucleus of living cells, thereby posing a mutagenic threat. For this purpose, C14-radiolabelled glutardialdehyde was infused rats; the animals were sacrificed after 5 minutes and 1 hour and liver samples were collected and examined for radioactivity distribution.
GLP compliance:
Type of method:
in vitro
Endpoint addressed:
other: subcellular localisation in liver cells

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Test material form:
Specific details on test material used for the study:
Glutaraldehyde, no further data given.

Test animals


Administration / exposure

Route of administration:
Details on study design:
C14-radiolabelled glutardialdehyde was infused rats; the animals were sacrificed after 5 minutes and 1 hour. Liver samples were collected and homogenized, and the extracellular material was removed by centrifugation. The remained components (cytosol, membrane, nucleus) were separated in fractions, and the volume, protein content and radioactivity of each fraction was determined for each of them.

Results and discussion

Details on results:
The findings showed that between 5 minutes and 1 hour, the cytosolic radioactivity content increased from 40 to 60% of the total tissue count whereas the membrane-bound radioactivity increased from 14 to 26%. The radioactivity related to the nuclear fraction was 0.5% of the total tissue count and remained stable between 5 minutes and 1 hour.

Any other information on results incl. tables

The finding of present study showed that glutaraldehyde administered i.v. to rats was incorporated in liver cells but did not reach the nucleus to a significant extent.

Applicant's summary and conclusion