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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term aquatic toxicity to invertebrates

EC50 (48h) = 0.058 mg/l

Toxicity to aquatic algae

ErC50 (72h) = 0.070 mg/l

Additional information

Short-term aquatic toxicity to invertebrates

The median effect concentration (EC50) and the no-effect concentration (NOEC) of INTERCURE 18 to Daphnia magna were investigated under static exposure conditions over a period of 48 h, following guideline OECD 202.

The investigated nominal concentrations were 0.100, 0.050, 0.025, 0.0125 and 0.00625 mg/l.

40 individual Daphnia divided into 2 test vessels were exposed to each concentrationof the test item, and 60 Daphnia divided into 3 test vessels were used as blank controls.

Under the test conditions, the substance shows an EC50 (48h) = 0.058 mg/l

Toxicity to aquatic algae

The effective concentrations (ErC10, ErC50and EyC10, EyC50) as well as the no observed effect concentrations (NOErC and NOEyC), of INTERCURE 18 to the green Desmodesmus subspicatus based on the average specific growth rate and the yield (algal biomass production) were investigated over a period of 72 h, according to the OECD guideline 201.

The test solutions were prepared by respective dilutions of a stock solution. The nominal concentrations were0.100, 0.0562, 0.0316, 0.0178 and 0.010 mg/l. Three parallel test vessels were used for each test concentration of the test item and six vessels for the blank controls.

Under the test conditions, the substance shows an ErC50 (72h) = 0.070 mg/l

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment (fish toxicity) when the following criteria are met:

A )Acute (short-term) aquatic hazard

Category Acute 1:

96 hr LC 50 (for fish) ≤ 1 mg/l and/or

48 hr EC 50 (for crustacea) ≤ 1 mg/l and/or

72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

B) Long-term aquatic hazard

(iii) Substances for which adequate chronic toxicity data are not available

Category Chronic 1:

96 hr LC 50 (for fish) ≤ 1 mg/l and/or

48 hr EC 50 (for crustacea) ≤ 1 mg/l and/or

72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

The EC50 value calculated for the test substance on the "short-term aquatic toxicity to invertebrates" (Daphnia) was established to be 0.058 mg/l, therefore the substance, for this test, is classified as Category Chronic 1 and Category Acute 1 ( 48 hr EC 50 (for crustacea) ≤ 1 mg/l) .

The ErC50 value calculated for the test substance on the "toxicity to aquatic algae and cyanobacteria" was established to be 0.070 mg/l. Even in this test the substance is classified in Category Chronic 1 and Category Acute 1 (72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l).