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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
: only males were tested, no pathologic examination was performed, lack of details on test substance
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-4-nitrobenzene
EC Number:
202-809-6
EC Name:
1-chloro-4-nitrobenzene
Cas Number:
100-00-5
Molecular formula:
C6H4ClNO2
IUPAC Name:
1-chloro-4-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: no data
- Weight at study initiation: 180-200 g
- Fasting period before study: no data
- Housing: 5 per cage
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data

Administration / exposure

Type of coverage:
semiocclusive
Vehicle:
polyethylene glycol
Details on dermal exposure:
Dermal application according to the method of Noakes and Sanderson, Brit. J. Industr. Med. 26, 1969, 59
Observation time: 14 days
Duration of exposure:
24 hours
Doses:
500, 600, 700, 900, 1000, 1200 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
750 mg/kg bw
Mortality:
600 mg/kg: 2 of 10 3 days after application
700 mg/kg: 5 of 10 3-4 days after application
900 mg/kg: 8 of 10 3-4 days after application
1000 mg/kg: 8 of 10 3-4 days after application
1200 mg/kg: 10 of 10 3h-3 days after application
Clinical signs:
sedation, cyanotic appearance, unkempt fur, palmospasm, low body temperature
Body weight:
not examined
Gross pathology:
not examined

Any other information on results incl. tables

Acute dermal toxicity of p-nitrochlorobenzene in rats

Dose (mg/kg bw)

Time of death

Dead

Symptoms

Number of rats used

500

600

700

900

1000

1200

-

3d

3-4d

3-4d

3-4d

3h-2d

0

2

5

8

8

10

10

10

10

10

10

10

10

10

10

10

10

10

Value: (670 - 830 mg/kg bw)

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Classification:
DSD: Xn, R21 Harmful in contact with skin
GHS: Acute Dermal Category 3 H311
Executive summary:

Loeser, Bayer AG, 1979

The study was performed comparable to guideline study 402 with acceptable restrictions (no pathologic examination was performed, lack of details on test substance). The acute dermal toxicity of 1-chloro-4-nitrobenzene was investigated in 10 male rats per dose according to the method of Noakes and Sanderson, 1969. The animals were dosed with 500, 600, 700, 900, 1000, 1200 mg/kg body weight. Sedation, cyanotic appearance, unkempt fur, palmospasm, low body temperature were observed at dose levels equal to and exceeding 600 mg/kg body weight. The LD50 was 750 mg/kg body weight.