Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Description of key information

P-chloronitrobenzene was found to be harmful if swallowed, in contact with skin and by inhalation.

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Dose descriptor:
294 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion
Dose descriptor:
discriminating conc.
16 100 mg/m³

Acute toxicity: via dermal route

Endpoint conclusion
Dose descriptor:
750 mg/kg bw

Additional information

p-chloronitrobenzene is toxic by oral administration, dermal contact and by inhalation.

The acute oral toxicity of 1 -chloro-4 -nitrobenzene was investigated in male Wistar II rats in a study comparable to OECD

guideline 401 with acceptable restrictions. 7 male groups of 10 animals were dosed with 100, 200, 300, 350, 400, 500 and 600 mg/kg bw 1-chloro-4-nitrobenzene per gavage and observed for 14 days following exposure for mortality and clinical signs. Mortalities occurred at dose levels equal to and exceeding 200 mg/kg bw 2 days after administration. Reduced general condition, cyanotic appearance, diarrhea, increased excretion of urine were observed in all male animals dosed 200 - 600 mg/kg bw. Symptoms started to appear between 3 hours (600 mg/kg bw) and 4 days (400 and 600 mg/kg bw) after administration. No clinical signs were observed in animals dosed with 100 mg/kg bw in male rats. The calculated LD50 for male rats was 294 mg/kg bw.

The dermal LD50 ranges for rats are between 750 - 1722 mg/kg body weight and the ranges in rabbit studies are between 2000 - 3160 mg/kg bw.

In a study performed comparable to guideline study 402 with acceptable restrictions the acute dermal toxicity of 1 -chloro-4 -nitrobenzene was investigated in male rats. The LD50 was 750 mg/kg body weight (Loeser, Bayer AG, 1979).

The acute inhalative toxicity of 1 -chloro-4 -nitrobenzene was investigated in study comparable to OECD guideline 403 with acceptable restrictions. Rats were exposed head-only to an atmosphere containing vapour and microcrystalline particles up to 16,100 mg/m³ air for 4 hours. Clinical signs of toxicity were related to dose. During and immediately after exposure cyanosis, corneal opacity, abnormal arched-back posture, lethargy, reddish-brown nasal and frothy mouth discharges, tachypnea, semi-prostration were observed. From 1 - 14 days post exposure pallor, lacrimation, alopecia, corneal opacity, stained perineal area, dermal irritations were observed. Aslo weight loss of 6 - 13 % was detected within the first 24 hours but weight gain normailzed therafter. Three days post exposure death occured at 16100 mg/m³ in 1 of 10 animals. Therefore the LCLo was 16100 mg/m³air (Dupont, 1981).

Justification for classification or non-classification

Based on the available study results, the following classification is derived; this deviates from the present legal classification (see Section 2):

LD50(oral) = 294 mg/kg bw


DSD: Xn, R22 Harmful if swallowed

GHS: Acute Oral Category 3 H301

LD50(dermal) = 750 mg/kg bw


DSD: Xn, R21 Harmful in contact with skin

GHS: Acute Dermal Category 3 H311

LCLo (inhalation) = 16100 mg/m3 air


DSD: Xn, R20 Harmful by inhalation

GHS: Acute Inhalation Category 3 H331