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EC number: 202-809-6 | CAS number: 100-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: National Guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Results of the harmful effects of water pollutants to green algae (Scenedesmus subspicatus) in the cell multiplication inhibition test
- Author:
- Kuehn R, Pattard M
- Year:
- 1 990
- Bibliographic source:
- Wat. Res. 24, 31-38
- Reference Type:
- secondary source
- Title:
- 1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.
- Author:
- OECD
- Year:
- 2 002
- Bibliographic source:
- UNEP Publications
Materials and methods
- Principles of method if other than guideline:
- DIN-Standard 38412 L9 (Algae, Cell multiplication inhibition test), Method of the German Standards Institution, Berlin, Germany
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1-chloro-4-nitrobenzene
- EC Number:
- 202-809-6
- EC Name:
- 1-chloro-4-nitrobenzene
- Cas Number:
- 100-00-5
- Molecular formula:
- C6H4ClNO2
- IUPAC Name:
- 1-chloro-4-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Before beginning the test, the stock solution of the test substance (sample) was adjusted to pH.
Test solutions
- Details on test solutions:
- The test substance was quantitatively dissolved to produce an stock solution in 800 ml double
distilled water.
Wide-neck bottles of 250ml with ground-glass stoppers were used as the test vessels.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Stock culture:
- Scenedesmus subspicatus (strain number 8681 SAG)
-100 ml flasks with caps and 20 ml nutrient solution sterilized and inoculated with 2 ml
cell suspension taken from a 10-day old culture.
Preliminary culture:
-The cell concentration in the preliminary culture flasks must amount to 10x E4 ml-1.
This was to ensure that the culture was still in a process of logarithmic growth after 72 h.
-The cell material of the preliminary cultures was used after 72 h to inoculate the dilution
preparation after the cell concentration had been fixed at 10xE5 ml-1.
- The cultivation of the preliminary cultures was undertaken 3 days prior to the preparation of the test solution.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 21-25°C
- pH:
- 8 ± 0.3
- Nominal and measured concentrations:
- Tested concentration range (mg/L; nominal): 0.4-50
- Details on test conditions:
- The test and control preparations were incubated under constant lighting and shaken daily.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Executive summary:
- Scenedesmus subspicatus proved to be a sensitive freshwater species in a cell multiplication inhibition test in accordance with DIN 38412, L9 (German Institute of Standardization). A 48 h-EC50 value (related to nominal concentration) of 16 mg/litre (growth rate) for Scenedesmus subspicatus was determined (Kuehn and Pattard, 1990).
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