1-chloro-4-nitrobenzene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: National Guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Results of the harmful effects of water pollutants to green algae (Scenedesmus subspicatus) in the cell multiplication inhibition test

Author:

Kuehn R, Pattard M

Year:

1990

Bibliographic source:

Wat. Res. 24, 31-38

Reference 2

Reference Type:

secondary source

Title:

1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.

Author:

OECD

Year:

2002

Bibliographic source:

UNEP Publications

Materials and methods

Principles of method if other than guideline:

DIN-Standard 38412 L9 (Algae, Cell multiplication inhibition test), Method of the German Standards Institution, Berlin, Germany

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Before beginning the test, the stock solution of the test substance (sample) was adjusted to pH.

Test solutions

Details on test solutions:

The test substance was quantitatively dissolved to produce an stock solution in 800 ml double
distilled water.
Wide-neck bottles of 250ml with ground-glass stoppers were used as the test vessels.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Stock culture:

- Scenedesmus subspicatus (strain number 8681 SAG)
-100 ml flasks with caps and 20 ml nutrient solution sterilized and inoculated with 2 ml
cell suspension taken from a 10-day old culture.

Preliminary culture:

-The cell concentration in the preliminary culture flasks must amount to 10x E4 ml-1.
This was to ensure that the culture was still in a process of logarithmic growth after 72 h.
-The cell material of the preliminary cultures was used after 72 h to inoculate the dilution
preparation after the cell concentration had been fixed at 10xE5 ml-1.
- The cultivation of the preliminary cultures was undertaken 3 days prior to the preparation of the test solution.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21-25°C

pH:

8 ± 0.3

Nominal and measured concentrations:

Tested concentration range (mg/L; nominal): 0.4-50

Details on test conditions:

The test and control preparations were incubated under constant lighting and shaken daily.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

4.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Executive summary:

Scenedesmus subspicatus proved to be a sensitive freshwater species in a cell multiplication inhibition test in accordance with DIN 38412, L9 (German Institute of Standardization). A 48 h-EC50 value (related to nominal concentration) of 16 mg/litre (growth rate) for Scenedesmus subspicatus was determined (Kuehn and Pattard, 1990).