Registration Dossier
Registration Dossier
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EC number: 203-907-1 | CAS number: 111-78-4
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity
- Author:
- Sparnins VL, Lam LKT and Wattenberg LW
- Year:
- 1 984
- Bibliographic source:
- Biochem. Biophys. Res. Commun. 120, 2, 637-640
Materials and methods
- Principles of method if other than guideline:
- The effects of cyclic 12-, 8- and 6-carbon compounds (incl. 1,5-cyclooctadiene) on the glutathione S-transferase (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the forestomach of female ICR/Ha mice were investigated.
- GLP compliance:
- no
- Type of method:
- in vivo
- Endpoint addressed:
- basic toxicokinetics
Test material
- Reference substance name:
- Cycloocta-1,5-diene
- EC Number:
- 203-907-1
- EC Name:
- Cycloocta-1,5-diene
- Cas Number:
- 111-78-4
- Molecular formula:
- C8H12
- IUPAC Name:
- (1Z,5Z)-cycloocta-1,5-diene
- Details on test material:
- 1,5-Cyclooctadiene from Aldrich Chemical Company, purity not reported
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ORGANISM
- Female ICR/Ha mice
- Age: 7 weeks
- Number: 5-10 animals per group
- Control: diet
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- TREATMENT - 30 or 50 µmol/g in diet for 2 weeks
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 2 weeks
- Frequency of treatment:
- every time
- Post exposure period:
- no
Doses / concentrations
- Remarks:
- Doses / Concentrations:
30 or 50 µmol/g diet
Basis:
nominal in diet
- No. of animals per sex per dose:
- 5 - 10
- Control animals:
- yes, plain diet
- Details on study design:
- no further details
Examinations
- Examinations:
- EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as substrate - Positive control:
- no
Results and discussion
- Details on results:
- None of the compounds elicited increased GST activity in the forestomach. C6 ring compounds showed no significant effect. C12 ring compounds
were more effective than C8 ring compounds with a decrease in activity in the order: unsaturated > epoxide > alcohol > saturated.
Any other information on results incl. tables
None of the compounds elicited increased GST activity in the forestomach.
C6 ring compounds showed no significant effect. C12 ring compounds were more
effective than C8 ring compounds with a decrease in activity in the order:
unsaturated > epoxide > alcohol > saturated.
-----------------------------------------------------------
Test compound Liver Small Bowel Mucosa
Specific Activity Specific Activity
------------------------------------------------------------
None 1.96 +- 0.16 0.61 +- 0.04
1,5-Cyclooctadiene 2.75 +- 0.26* 0.76 +- 0.03*
* = p <0.005
Applicant's summary and conclusion
- Conclusions:
- 1,5-cyclooctadiene was few effective in producing increased GST activity in the liver.
- Executive summary:
Glutathione S-transferase is considered as a major detoxification system which catalyzes conjugation of electrophilic compounds, e.g. chemical carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon compounds (incl. 1,5-cyclooctadiene) on the
glutathione S-transferase (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the forestomach of female ICR/Ha mice were investigated.
None of the compounds elicited increased GST activity in the forestomach. C6 ring compounds showed
no significant effect. C12 ring compounds were more effective than C8 ring compounds with a decrease in activity in the order: unsaturated > epoxide > alcohol > saturated.
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