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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: sufficient documented and scientifically acceptable
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Principles of method if other than guideline:
100 µl of the liquefied test substance (at 30°C) was applied into the conjunctival sac of 2 rabbits. the post-observation time was 7 days
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dichloro-5-isocyanatobenzene
EC Number:
252-276-9
EC Name:
1,3-dichloro-5-isocyanatobenzene
Cas Number:
34893-92-0
Molecular formula:
C7H3Cl2NO
IUPAC Name:
1,3-dichloro-5-isocyanatobenzene
Details on test material:
no data

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Duration of treatment / exposure:
see observation period
Observation period (in vivo):
7 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 0, 24, 48 h, 5, 6, and 7 d
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 0, 24, 48 h, 5, 6, and 7 d
Score:
0
Max. score:
2
Irritation parameter:
other: erythema
Basis:
mean
Time point:
other: 0, 24, 48 h
Score:
1.7
Max. score:
4
Reversibility:
not fully reversible within: 7 d
Irritation parameter:
other: erythema
Basis:
mean
Time point:
other: 5, 6, 7 d
Score:
1
Max. score:
4
Reversibility:
not fully reversible within: 7 d
Irritation parameter:
other: edema
Basis:
mean
Time point:
other: 0, 24, 48 h
Score:
1
Max. score:
4
Irritation parameter:
other: edema
Basis:
animal #1
Time point:
other: 5, 6, 7 d
Score:
1
Max. score:
4
Irritation parameter:
other: edema
Basis:
animal #2
Time point:
other: 5, 6, 7 d
Score:
0
Max. score:
4

Any other information on results incl. tables

Up to 24 h a mildly reddening of the conjunctivae and an slightly edema was seen. Afterwards the conjunctivae of 1 animal was mildly reddened during the post-observation period. The other animal revealed form day 5 to 7 of the post-observation period a mildly reddening of the conjunctivae but no edema.

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Executive summary:

method: 100 µl of the liquedfied test substance (at 30°C) was applied into the conjunctival sac of 2 rabbits.

result: substance is slightly irritating. No adverse effects on cornea and iris were seen during the post-observation period in both animals.

During the post-observation time the erythema and edema scores of both amimals were below 2.

Therefore a classification is not justified.