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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH (Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation :average 1.63 cm
- Weight at study initiation (mean and range, SD):0.1899g (average)
ACCLIMATION- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
148.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.05
Dissolved oxygen:
8.0 mg/l
Nominal and measured concentrations:
control and 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: The test vessels used were all plastic aquaria having the water capacity of 5liters.
fill volume: 2 liters
- No. of organisms per vessel:8 fishes
OTHER TEST CONDITIONS - Photoperiod:16 hours light and 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

 

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24 h

48h

72h

96h

Control

4

4

8.16

8.17

8.01

8.07

7.95

7.99

7.66

7.74

100

4

4

8.36

8.37

8.12

8.13

8.27

8.30

7.52

7.57

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24 h

48h

72h

96h

Control

4

4

7.01

7.01

6.99

6.99

5.99

5.99

6.21

6.27

100

4

4

6.96

6.96

6.86

6.86

5.83

5.89

5.83

5.87

Test temperature

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24 h

48h

72h

96h

Control

4

4

23.1

23.2

23.9

23.9

23.9

23.9

23.9

23.8

100

4

4

23.4

23.4

23.7

23.7

23.5

23.4

23.2

23.1

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on ZEBRA FISH(Danio rerio)was determine to be >100 mg/L.
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Based on the data obtain from various sources for the target chemical and for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical on the mortality of fishes. The studies are as mentioned below:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

First study was supported by the second from experimental report. This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio). 5 liters Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 06-11-2017 and lasted for a period of 96 hrs. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L, 25mg/L, 50mg/L,100mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After completion of stirring test solution prepare for achieving test concentrations. No effect were observed at 100 mg/l nominal concentration. After 96 hours of exposure of test itemTetraethylammonium Chloride on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra Fish Danio rerio was determine to be > 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Thus based on the overall studies it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.