Amines, C12-14-alkyl, isooctyl phosphates

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20.03.2000 to 19.07.2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

GLP guideline study with deviation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

yes

Remarks:

Pre adaption of the inoculum

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Amines, C12-14-alkyl, isooctyl phosphates

EC Number:

269-119-5

EC Name:

Amines, C12-14-alkyl, isooctyl phosphates

Cas Number:

68187-67-7

Molecular formula:

C19H42NPO4 - C29H63NPO4

IUPAC Name:

Amines, C12-14-tert-alkyl, isooctyl mono phosphates

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

Mixed population of aquatic micro organisms (activated sludge) collected on 20.06.2000 from the aeration tanks (step II) of the joint sewage treatment plant Bürrig. The inoculum was adapted to 100mg/l of the test substance for approximately 4 weeks before the start of the study.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The test substance was suspended in a mineral medium, inoculated with a mixed population of aquatic micro organism (30mg ss/l) and incubated for 28 days under aerobic conditions in the dark at 20± 1°C. During this period the biodegradation of the test material was determined on days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28 by measuring the oxygen consumption with a respirometer.

TEST SET-UP:
- Blank: test medium only (3 replicates)
- Inoculum control: test medium and added reference substance sodium benzoate 100 mg/l (3 replicates)
- Test substance: test medium and added test substance 100 mg/l (3 replicates)
- Toxicity control: test medium added test substance (100 mg/l) and reference substance (100 mg/l). (2 replicates)

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/l

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

35

Sampling time:

28 d

Details on results:

For the reference substance sodium benzoate biodegradation of 81% was acheived in 14 days.

See attached background information

Any other information on results incl. tables

See attached background information

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

other: not readily biodegradable

Conclusions:

The test item attained 35% degradation after 28 day and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of the guidelines.

Executive summary:

The test was conducted to according to the Manometric Respiratory test Method Council Directive 92/69 EEC Method C.4 -D.

The test substance was degraded by 35% after 28 days.