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EC number: 200-164-5 | CAS number: 53-16-7
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- Ecotoxicological Summary
- Aquatic toxicity
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- Short-term toxicity to fish
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Inducibility of chromosome aberrations by steroid hormones in cultured chinese hamster ovary cells
- Author:
- Kochhar T.S.
- Year:
- 1 985
- Bibliographic source:
- Toxicology Letters 29, 201-206 (1985)
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- CHO cells used in the current investigation were routinely grown in 75cm² Corning plastic flasks in 10 ml of McCoy’s 5a medium supplemented with 10% fetal calf serum and antibiotics consisting of penicillin-G, (100 U/ml) and streptomycin, (100 µg/ml). The cultures were incubated in an atmosphere of 5% CO2 in air. To test the effect of steroid hormones, cultures were set up 24 h prior to the treatment by seeding approx. 1.6 x 10 to 6 cells per flask. Treatments were given by exposing the cultures to test compounds dissolved in DMSO and suspended in the culture medium for 12 h. The control cultures contained 0.5% of DMSO and were incubated under identical conditions. The procedure for the preparation of chromosomes to score for the abnormalities
is described in Experientia, 38 (1982) 845-846. - GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Estrone
- EC Number:
- 200-164-5
- EC Name:
- Estrone
- Cas Number:
- 53-16-7
- Molecular formula:
- C18H22O2
- IUPAC Name:
- estrone
Constituent 1
- Specific details on test material used for the study:
- The test material was procured from Sigma, St. Louis, MO.
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- The CHO cells were obtained from Environmental Health Research and Testing, Lexington, KY. The cells were not used at a passage level of more than 15 after cloning, and were thawed routinely from liquid nitrogen storage and maintained by transferring twice a week.
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- 10 to minus five M; 5 x 10 to minus five M; 10 to minus 4 M
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- no
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- not applicable
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- not examined
Applicant's summary and conclusion
- Conclusions:
- positive
- Executive summary:
The effect of steroid hormones on the chromosomes of cultured Chinese hamster ovary (CHO) cells was studied. It was noticed that estradiol-17fl, estrone, estriol and ethynyl estradiol were effective in producing various types of chromosome aberrations. The percentage of these abnormalities increased with increasing concentrations of steroids used.
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