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Toxicity to terrestrial plants

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Reference
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019-2020
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants, Growth Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Potasum hexatitanate, potassium titanium oxide
Batch n. MA-95150
Solid, light-yellow powder
Analytical monitoring:
not required
Remarks:
The analytical monitoring of the test item was not required, according to the test guideline.
Vehicle:
yes
Remarks:
fine quartz sand (as dispersant)
Details on preparation and application of test substrate:
The test item was mixed with fine quartz sand (as dispersant) and then applied with this mixture into soil. Application was done with laboratory mixer. The seeds were afterwards sown in the treated soil.
Untreated control was obtained with the same amount of untreated quartz sand as in the test item treated groups and added to the soil.
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Species:
Lycopersicon esculentum
Plant group:
Dicotyledonae (dicots)
Species:
Cucumis sativus
Plant group:
Dicotyledonae (dicots)
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Test type:
seedling emergence and seedling growth test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
yes
Total exposure duration:
21 d
Remarks:
A preliminary range finder test was performed, that showed no effects on fresh weight. Therefore, the limit test was choosen.
Test temperature:
22 °C ± 10 °C
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): commercial plastic flower pots (15 cm diameter)
- No. of seeds per container:

Species Seeds per Pot Replicates (No of pots) Tested Seeds per Treatment Group
Brassica napus 3 7 21
Glycine max 2 10 20
Solanum lycopersicum 2 10 20
Cucumis sativus 2 10 20
Avena sativa 5 4 20
Allium cepa 5 4 20


SOURCE AND PROPERTIES OF SUBSTRATE
The soil was steam sterilized, analysed and delivered by the Landwirtschaftliche Untersuchungs- und Forschungsanstalt (LUFA) Speyer: LUFA 2.3 (USDA: sandy loam), all particles =< 0.2 cm, organic carbon maximum 1.5%. No pesticide treatment was performed in the year of sampling and 2 previous years.

GROWTH CONDITIONS
- Photoperiod: 16 hrs light, 8 hrs dark
- Light intensity: minimum 200 microE/m2/s

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test, 1000 mg/kg soil dw

RANGE FINDING STUDY

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Species Pot Treatment group (mg test item/kg dry soil)
Brassica napus control 0,10 1,00 10,0 100 1000
1 12,293 11,119 10,084 12,015 12,195 10,295
2 11,788 11,261 10,624 11,940 12,537 12,605
3 11,220 12,311 11,529 11,411 11,202 9,035
Mean [g] 11,767 11,564 10,746 11,789 11,978 10,645
Standard Deviation ± 0,54 ± 0,65 ± 0,73 ± 0,33 ± 0,69 ± 1,81
% of Control 98,3 91,3 100,2 101,8 90,5
Effect [%] -1,7 -8,7 0,2 1,8 -9,5

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Species Pot Treatment group (mL oder mg test item oder a.i./ha oder kg)
Glycine max control 0,10 1,00 10,0 100 1000
1 7,849 9,246 10,676 9,523 8,492 10,177
2 8,435 11,297 7,349 9,314 8,079 10,450
3 9,073 12,125 8,818 12,737 9,982 9,509
Mean [g] 8,452 10,889 8,948 10,525 8,851 10,045
Standard Deviation ± 0,61 ± 1,48 ± 1,67 ± 1,92 ± 1,00 ± 0,48
% of Control 128,8 105,9 124,5 104,7 118,8
Effect [%] 28,8 5,9 24,5 4,7 18,8

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Species Pot Treatment group (mL oder mg test item oder a.i./ha oder kg)
Solanum lycopersicum control 0,10 1,00 10,0 100 1000
1 1,418 1,835 2,005 2,404 1,630 2,024
2 1,968 1,880 1,881 1,747 1,874 1,078
3 2,020 1,723 1,961 1,924 1,824 2,286
Mean [g] 1,802 1,813 1,949 2,025 1,776 1,796
Standard Deviation ± 0,33 ± 0,08 ± 0,06 ± 0,34 ± 0,13 ± 0,64
% of Control 100,6 108,2 112,4 98,6 99,7
Effect [%] 0,6 8,2 12,4 -1,4 -0,3

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Species Pot Treatment group (mL oder mg test item oder a.i./ha oder kg)
Cucumus sativus control 0,10 1,00 10,0 100 1000
1 7,472 5,297 7,032 4,429 6,568 5,566
2 5,553 7,537 5,248 6,551 6,666 6,534
3 5,727 7,238 7,360 6,448 5,645 4,894
Mean [g] 6,251 6,691 6,547 5,809 6,293 5,665
Standard Deviation ± 1,06 ± 1,22 ± 1,14 ± 1,20 ± 0,56 ± 0,82
% of Control 107,0 104,7 92,9 100,7 90,6
Effect [%] 7,0 4,7 -7,1 0,7 -9,4

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Species Pot Treatment group (mL oder mg test item oder a.i./ha oder kg)
Avena sativa control 0,10 1,00 10,0 100 1000
1 3,414 3,544 4,771 4,683 4,721 5,725
2 3,930 3,734 4,556 4,313 4,369 4,293
3 4,359 3,951 5,023 5,041 4,628 4,315
Mean [g] 3,901 3,743 4,783 4,679 4,573 4,778
Standard Deviation ± 0,47 ± 0,20 ± 0,23 ± 0,36 ± 0,18 ± 0,82
% of Control 95,9 122,6 119,9 117,2 122,5
Effect [%] -4,1 22,6 19,9 17,2 22,5

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Species Pot Treatment group (mL oder mg test item oder a.i./ha oder kg)
Allium cepa control 0,10 1,00 10,0 100 1000
1 0,579 0,701 0,577 0,499 0,502 0,673
2 0,679 0,787 0,635 0,621 0,606 0,712
3 0,621 0,672 0,683 0,444 0,736 0,688
Mean [g] 0,626 0,720 0,632 0,521 0,615 0,691
Standard Deviation ± 0,05 ± 0,06 ± 0,05 ± 0,09 ± 0,12 ± 0,02
% of Control 115,0 100,9 83,2 98,1 110,3
Effect [%] 15,0 0,9 -16,8 -1,9 10,3

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Nominal and measured concentrations:
1000 mg/kg soil dw
Key result
Species:
Brassica napus
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Glycine max (G. soja)
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Lycopersicon esculentum
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Cucumis sativus
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Avena sativa
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Allium cepa
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Key result
Species:
Brassica napus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Key result
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Key result
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Key result
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Key result
Species:
Avena sativa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Key result
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality
Details on results:
At 1000 mg/kg soil dw:
- seedling emergence was >= 85% in all tested species.
- mortality was 0% in all tested species

At 1000 mg/kg soil dw fresh weigth effects were observed as follows:
- Brassica napus -4.2%
- Glycine max -3.3%
- Lycopersicon esculentum -19.5%
- Cucumis sativus -13.7%
- Avena sativa +20.1%
- Allium cepa -8.5%
Validity criteria fulfilled:
yes
Conclusions:
The study did not show adverse effects of the substance on terrestrial plants.

Description of key information

No significant effects were observed. NOEC values are >= 1000 mg/kg soil dw.

Key value for chemical safety assessment

Additional information