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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two plankton crustaceans: the key role of organism-water partitioning
Author:
Abernethy S, Bobra AM, Shiu WY, Wells PG, Mackay D
Year:
1986
Bibliographic source:
Aquatic Toxicology 8, 163-174

Materials and methods

Principles of method if other than guideline:
Toxicity test according to Bobra et al. (1983), Chemosphere 12, 1137-1149.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroform
EC Number:
200-663-8
EC Name:
Chloroform
Cas Number:
67-66-3
Molecular formula:
CHCl3
IUPAC Name:
chloroform
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Saturated aqueous solutions of chloroform were prepared and diluted to provide at least 5 exposure concentrations plus a control. Test chambers were filled and sealed in glass vials with teflon-lined screw caps. Air spaces in exposure chambers were eliminated to minimise volatilisation loss of the toxicant from water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
4 to 6 day old Daphnia from laboratory-breeded stocks were used for the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
23 +/- 2 °C
Dissolved oxygen:
Concentration was typically at 8 to 9 mg/L and after 48 hours, the lowest measured concentration was 5 mg/L.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
79 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality

Applicant's summary and conclusion

Executive summary:

The acute toxicity of chloroform to 4 to 6 day old Daphnia magna was tested in a static test with sealed vessels. The endpoint for the toxicity assessment was mortality. The LC50 value determined was 79 mg/L.