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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No OECD guideline or GLP defined

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity Test Data of Existing Chemical Substances; 2,4-Dichlornitrobenzene; Bacterial Mutagenicity Test
Author:
Japan Chemical Industry Ecology-Toxicology and Information Center, Japan (JETOC)
Year:
1996
Bibliographic source:
JETOC

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: guidelines of Ministry of Labour, Japan (1979, 1985 and 1988); methods of Ames et al. (1975), Maron and Ames (1983) and Matsushima et al. (1980).
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Method

Target gene:
ames assay: detection of base pair substitutions and frameshift mutations
Species / strain
Species / strain / cell type:
other: TA98, TA100, TA102, TA1535 and TA1538, Escherichia coli WP2uvrA/pKM101
Metabolic activation:
with and without
Test concentrations with justification for top dose:
see: Any other information on materials and methods incl. tables
Controls
Positive controls:
yes
Remarks:
see: Any other information on materials and methods incl. tables

Results and discussion

Test results
Species / strain:
other: TA98, TA100, TA102, TA1535 and TA1538, Escherichia coli WP2uvrA/pKM101
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

 Con.µg/plate                             Number of Revertants/plate
   Base-substitution                          Flame-shift
   TA100     TA1535     WP2uvrA     TA98       TA1537 
 S9-  S9+   S9-   S9+   S9-   S9+   S9-   S9+   S9-   S9+
 DSMO  (133) (142)  (12)  (16)  (19)  (21)  (16)  (18)  (7)  (9) 
   135  125 17   20  29  18  15  14  9  9
   146  141  7  7  24  32  12  23  7  8
 0.0763  (141) (133)  (12)  (14)  (27)  (25)  (14)  (19)  (8)  (9) 
   157  116  13  15  16  15  14  18  14  12
   158  132  21  13  18  25  13  23  9  7
 0.305  (158) (124)  (17)  (14)  (17)  (20)  (14)  (21)  (12)  (10) 
   169  141  7  8  7  25  24  27  14  13
   146  197  13  16  16  27  21  29  8  12
 1.22  (158) (169)  (10)  (12)  (12)  (26)  (23)  (28)  (11)  (13) 
   135 190   14  21  24  30  32  25  5  8
   169  198  7  18  22  15  16  22  13  10
 4.88  (152) (194)  (11)  (20)  (23)  (23)  (24)  (24)  (9)  (9) 
   149  198  14  16  17  24  12  35  6  5
   154  201  9  14  17  25  17  25  9  8
 19.5  (152)  (200) (12)  (15)  (17)  (25)  (15)  (30)  (8)  (7) 
   185  417  14  17  23 12   14  29  7  12
   168  401  15  14  29  27  21  32  5  7
 78.1  (177)  (409)  (15)  (16)  (26)  (20)  (18)  (31)  (6)  (10)
   0*   0*  0*   0*   0*  40*  0*   0*   0*   0*
  0*    0*   0*   0*   0*  13*  0*   0*   0*   0*
 313  (0*)    (0*)   (0*)     (0*)   (0*)   (27*)    (0*)   (0*)     (0*)    (0*)
 1250    (0*)   (0*)    (0*)     (0*)   (0*)     (0*)    (0*)    (0*)    (0*)   (0*) 
 5000    (0*)   (0*)     (0*)   (0*)     (0*)   (0*)     (0*)   (0*)     (0*)   (0*) 
 Judgment  -  +  -  -  -  -  -  -  -  -
 Specific mutagenicity    3420                
 Positive control  AF2  2AA  NaN3  2AA  AF2  2AA  AF2  2AA  9AA  2AA
   (807)  (928)  (258)  297  (190)  (780)  (493)  (310)  (342)  (187)

Applicant's summary and conclusion

Executive summary:

The test substance was tested in an Ames Salmonella/ microsome assay. The bacterial strains used were: TA98, TA100, TA102, TA1535, TA1537, TA1538 and Escherichia coli WP2uvrA and WP2uvrA/pKM101. The test substance was tested without metabolic activation and with S-9 mix from male Sprague-Dawley rats.

The following standard mutagens served as positive controls:

AF2: 2 -(2 -furyl)-3 -(5 -nitro-2 -furyl)acrylamide, NaN3: sodium azide, 9AA: 9 -aminoacridine, 2NF: 2 -nitrofluorene, 4NQO: 4 -nitroquinoline-N-oxide, BLM: bleomycin, PA: pyruvic aldehyde, 2AA: 2 -amino anthracene.

Al chemicals except BLM and PA were dissolved in DMSO. BLM and PA were dissolved in sterile distilled water.

The test substance was found to be mutagenic only for strain  TA 100 with  metabolic activation.