2,3,5-trimethylphenol

Endpoint summary

Description of key information

An experimental study conducted  by Tetsuo Yamano et al.( Toxicology , 2007) on target substance2,3,5 trimethylphenols (697-82-5) on mice. LLNA test was conducted on male BALB/cA mice for 2,3,5 trimethylphenols (697-82-5) . On 3 consecutive days the groups of 3 mice were exposed to at 25 µl of 1M of chemical. Acetone/ olive oil, 4:1 were used as a vehicle. Three or five days after the last application, a pair of auricular lymph nodes (LNs) from each mouse was excised for the LLNA. A stimulation index was calculated as the ratio of the BrdU amount in the lymph nodes of chemical-treated mice to that of vehicle-treated mice. Stimulation index variation was observed as 0 for the test chemical.From experiment it was concluded that the 2,3,5Trimethylphenol (697-82-5) was considered to be non-sensitizing in mice LLNA assay.

Key value for chemical safety assessment

Skin sensitisation

Link to relevant study records

Reference

Endpoint:

skin sensitisation: in vivo (LLNA)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from publication.

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Mouse local lymphnode assay (LLNA) was used to determined skin sensitization potential of 2,3,5Trimethylphenol in mice.

GLP compliance:

not specified

Type of study:

mouse local lymph node assay (LLNA)

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2,3,5Trimethylphenol
- Molecular formula (if other than submission substance): C9H12O
- Molecular weight (if other than submission substance): 136.193 g/mol
- Substance type: Organic
- Physical state: Solid

Species:

mouse

Strain:

other: BALB/cA mice

Sex:

male

Details on test animals and environmental conditions:

Details on test animal
TEST ANIMALS
- Source: CLEA Japan (Tokyo, Japan)
- Age at study initiation: Six- to eight-week-old
- Weight at study initiation: No data available
- Housing: No data available
- Diet (e.g. ad libitum): No data available
- Water (e.g. ad libitum): No data available
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available

Vehicle:

other: acetone/ olive oil, 4:1, AOO

Concentration:

25 µl of 1M of 2,3,5 trimethylphenols

No. of animals per dose:

3 mice

Details on study design:

TREATMENT PREPARATION AND ADMINISTRATION: Groups of three mice were exposed to 25 µl of 1M methylphenols, 0.3% DNCB, 3% TMA, or vehicle (acetone/ olive oil, 4:1, AOO) through application to the dorsum of both ears for 3 consecutive days. Three or five days after the last application, a pair of auricular lymph nodes (LNs) from each mouse was excised for the LLNA

Positive control substance(s):

not specified

Statistics:

The values for each chemical-treated group and vehicle group (control group) were compared via Dunnett’s or Steel’s multiple comparison method using a StatLight software package

In stimulation index was calculated as the ratio of the BrdU amount in the lymph nodes of chemical-treated mice to that of vehicle-treated mice.

Key result

Parameter:

SI

Value:

0 - 0

Variability:

1-12

Test group / Remarks:

Stimulation index result was zero for the test group.

Remarks on result:

other: No indication of skin sensitization .

Cellular proliferation data / Observations:

Yes

No indication of skin sensitization .

Interpretation of results:

other: not sensitizing

Conclusions:

Skin sensitization test was observed for 2,3,5 trimethylphenols (697-82-5) in BALB/cA mice by LLNA method.The test result for 2,3,5Trimethylphenol (697-82-5) was in LLNA assay.

Executive summary:

LLNA test was conducted on male BALB/cA mice for 2,3,5 trimethylphenols (697-82-5) . On 3 consecutive days the groups of 3 mice were exposed to at 25 µl of 1M of chemical. Acetone/ olive oil, 4:1 were used as a vehicle. Three or five days after the last application, a pair of auricular lymph nodes (LNs) from each mouse was excised for the LLNA. A stimulation index was calculated as the ratio of the BrdU amount in the lymph nodes of chemical-treated mice to that of vehicle-treated mice. Stimulation index variation was observed as 0 for the test chemical.From experiment it was concluded that the 2,3,5Trimethylphenol (697-82-5) was considered to be non-sensitizing in mice LLNA assay.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not sensitising)

Additional information:

Skin sensitization

In different studies, 2,3,5 trimethylphenols (697-82-5) has been investigated for potential for dermal sensitization to a greater or lesser extent. The study is based on in vivo experiments in mice by LLNA method for target chemical2,3,5 trimethylphenols (697-82-5). The target experimental data has also been supported with predicted data using the OECD QSAR toolbox.

A key experimental study conducted  by Tetsuo Yamano et al.( Toxicology , 2007) on target substance2,3,5 trimethylphenols (697-82-5) on mice. LLNA test was conducted on male BALB/cA mice for 2,3,5 trimethylphenols (697-82-5) . On 3 consecutive days the groups of 3 mice were exposed to at 25 µl of 1M of chemical. Acetone/ olive oil, 4:1 were used as a vehicle. Three or five days after the last application, a pair of auricular lymph nodes (LNs) from each mouse was excised for the LLNA. A stimulation index was calculated as the ratio of the BrdU amount in the lymph nodes of chemical-treated mice to that of vehicle-treated mice. Stimulation index variation was observed as 0 for the test chemical.From experiment it was concluded that the 2,3,5Trimethylphenol (697-82-5) was considered to be non-sensitizing in mice LLNA assay.

The skin sensitization potential of 2,3,5 trimethylphenols (697-82-5) was estimated by SSS (2017) using OECD QSAR toolbox v3.3 with log kow as the primary descriptor and considering the six closest read across substances. 2,3,5 trimethylphenols (697-82-5) was predicted to be non sensitizing to the skin of Hartley strain and Hartley-English short hair cross-strain guinea pig.

 

Thus based on the experimental study and prediction on 2,3,5 trimethylphenols (697-82-5) , it can be concluded that 2,3,5 trimethylphenols is not a skin sensitizer. Thus comparing the above annotations with the criteria of CLP regulation, 2,3,5 trimethylphenols (697-82-5) can be considered as not classified for skin sensitization effects.

Justification for classification or non-classification

Thus comparing the above annotations with the criteria of CLP regulation, 2,3,5 trimethylphenols (697-82-5) can be considered as not classified for skin sensitization effects