3-methyl-2-pent-2-enylcyclopent-2-enone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 September 2014 - 17 October 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Preparation of test solutions started with 100 mg/l, applying a 16 to 18-minute period of magnetic stirring to accelerate the dissolving of the test substance in the test medium. This concentration was prepared in two (range-finding test) or three (final test) batches that were subsequently mixed and used as the highest concentration. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless.
Because the test substance was volatile and sensitive to light, all handlings were performed in closed vessels and under dimmed light.

Vehicle:

no

Details on test solutions:

The batch of the test item was a clear colourless liquid completely soluble in test medium at the concentrations tested.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Strain: Cyprinus carpio
- Length at study initiation: 2.4 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.40 ± 0.09 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water

ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions: Adjusted ISO medium
- Type of food during acclimation: pelleted fish food
- Feeding frequency during acclimation: Daily
- Health during acclimation (any mortality observed): mortality during the seven days prior to the start of the test was less than 5%

FEEDING DURING TEST
No feeding from 24 hours prior to the test and during the total test period

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg CaCO3 per litre

pH:

7.7 ± 0.3

Nominal and measured concentrations:

Nominal: 4.6, 10, 22, 46 and 100 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass
- Type: closed
- Material, size, headspace, fill volume: 6 litres
- Aeration: not aerated during the test
- No. of organisms per vessel: 7 per concentration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.47 g fish/litre

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and clinical effects

TEST CONCENTRATIONS:
- Range finding study
- Test concentrations: 0.1 to 100 mg/l increasing by a factor of 10.

Reference substance (positive control):

yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

54 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

54 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

weight

Details on results:

The actual concentrations were at the level of 94-109% of nominal and remained stable during the test (93-101% of initial). Therefore, the effect parameters were expressed in terms of nominal concentrations.

Results with reference substance (positive control):

Study period: 13 January 2014 - 17 January 2014
Concentrations: 0.10, 0.22 and 0.46 mg/l in ISO-medium.
Reference substance: pentachlorophenol
Under the conditions of the present test the reference substance induced no lethal effects in carp at 0.10 mg/l. The 96h-LC50 for carp exposed to the reference substance was 0.24 mg/l. This effect was already reached within 48 hours of exposure.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study the 96h-LC50 was 54 mg/l based on nominal concentrations (95% confidence interval could not be determined).

Description of key information

LC50 (96 h) = 54.0 mg/L (nominal, OECD 203, C. carpio)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

54 mg/L

Additional information

There is one study available, in which the short-term toxicity of (Z)-3-methyl-2-(pent-2-en-1-yl)cyclopent-2-enone (CAS 488-10-8) to fish was assessed according to the OECD guideline 203 and GLP.

In a static test, Cyprinus carpio was exposed to the nominal test item concentrations of 4.6, 10, 22, 46, and 100 mg/L for 96 h. The test item concentrations were analytically verified by GC at the start and end of the test.

The measured concentrations were 94 – 109% of nominal at the start of the test and remained stable throughout the test (93 – 101% of initial at test end). Therefore, the effect parameters were expressed in terms of nominal concentrations. After 96 h mortality was observed and an LC50 (96 h) of 54 mg/L was determined (95% confidence interval could not be determined).