Reaction product of diazotised 4-amino-5-hydroxy-2,7- naphthalenedisulfonic acid coupled with resorcinol, subsequently coupled with diazotised 2-amino-4,6-dinitrophenol and 4-nitrobenzenamine, chelated with iron (3+), sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 17, 2000 to March 16, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

other: Royal Decree 363/1995 (BOE no. 133 of June 1995).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss (Cladocera, Crustacea)
- Source: University of Stirling UK
- Age at study initiation: < 24 hours
- Feeding during test: no
- Food type: Chlorella (freshwater microalgae) and seaweed extract.

ACCLIMATION
- Temperature: 18 - 22 ºC
- Type of food: Chlorella (freshwater microalgae) and seaweed extract.
- Feeding frequency: three time per week.
- Photoperiod: 16 hours light / 8 hours of darkness.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 2 ºC

pH:

7.96 - 7.97

Dissolved oxygen:

8.08 - 8.21 mg/l O2

Nominal and measured concentrations:

NOMINAL: 0.1 mg/l, 1 mg/l, 10 mg/l, 100 mg/l and 1000 mg/l
MEASURED: 0.1 mg/l, 1 mg/l, 10 mg/l, 100.1 mg/l and 1000.8 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 40 ml tube
- Type (delete if not applicable): closed
- Material, size: 10 ml
- Aeration: no, closed with parafilm.
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- Photoperiod: continuous darkness.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD 202
— Solution 1: CaCl 2 x 2H2O 11,76 g/l H2O
— Solution 2: MgSO4 x 7H2O 4,93 g/l H2O
— Solution 3: NaHCO3 2,59 g/l H2O
— Solution 4: KCl 0,23 g/l H2O

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.03 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24 h) = 1,1 mg/l

Inhibition of mobility

Conc. mg/l	% Inhibition mobility (48h)
Control	0
0.1	15
1	55
10	85
100	90
1000	100

Conclusions:

The substance was tested for short term toxicity to invertebrates following OECD 202. Under the experimental conditions the EC50 (48 h) equal to 1.03 mg/l, nominal

Executive summary:

The substance has been tested for short-term toxicity on freshwater invertebrates according to the guideline OECD 202 (1984) and Royal Decree 363/1995 (BOE no.133 of June 1995).
The freshwater crustacean Daphnia magna has been subjected to the dye at concentrations of: 0.1mg/L, 1 mg/L, 10 mg/L, 100mg/L and 1000 mg/L of active ingredient of that product. The total test duration was 48 hours.
The result obtained in this test indicates that the dye had a nominal value of EC50 (48 h) equal to 1.03 mg/L.

Description of key information

EC50 (48 h) = 1.03 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.03 mg/L

Additional information

OECD 202 (1984)