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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 17, 2000 to March 16, 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
other: Royal Decree 363/1995 (BOE no. 133 of June 1995).
GLP compliance:
no
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Brachydanio rerio
- Source: P & S, S.L
- Length at study initiation: 2.75 ± 0.13 cm
- Weight at study initiation: 0.27 ± 0.1 g
- Feeding during test: no data

ACCLIMATION
- Acclimation period: 12 days
- Type and amount of food: commercial food
- Feeding frequency: 3 times per week up to 24 hours before the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
115 mg CaCO3/l
Test temperature:
22 ± 2 °C
pH:
8,09
Dissolved oxygen:
> 60%
Nominal and measured concentrations:
NOMINAL: 1 mg/l, 3.2 mg/l, 10 mglL, 32 mg/l and 100 mg/l
MEASURED: 1 mg/l, 3.21 mg/l, 10 mg/l, 32.1 mg/l and 100.4 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 3 l glass aquarium
- Type (delete if not applicable): closed
- Renewal rate of test solution: after 48 hours.
- Aeration: continuous aeration.
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1

OTHER TEST CONDITIONS
- Temperature: 22 ± 2 °C
- Photoperiod: 12 hours darkness.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality

Number of fish dead during the test period:

Concentration mg/l Mortality
time 0h 24h 48h 72h 96h
Control  0 0 0 0 0
1 0 0 0 0 0
3.2 0 0 0 0 0
10 0 0 0 0 0
32 0 0 0 0 0
100 0 0 0 0 0
Conclusions:
The LC50(96h) is more than 100 mg/L based on active ingredient.
Executive summary:

The substance has been tested for short-term toxicity testing on fish according to the guideline OECD 203 (1992) and Royal Decree operating method 363/1995 (BOE no.133 of June 1995).
The freshwater fish Brachydanio rerio has been subjected to the dye at test concentrations of: 1 mg/l, 3.21 mg/l, 10 mg /l, 32.1 mg/l
and 100.4 mg/l. The total test duration was 96 hours. The nominal LC50 (96 h) is more than 100 mg/L based on active ingredient.

Description of key information

LC50 (96 h) > 100 mg/l 

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

OECD 203 (1992)