Registration Dossier
Registration Dossier
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EC number: 274-999-9 | CAS number: 70900-27-5
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48 h EC50 was >100 mg/L and the 48 h NOEC was 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The acute toxicity of the test material to Daphnia magna was investigated in a study conducted in accordance with the standardised guidelines OECD 202, EU Method C.2, the US EPA OPPTS 850.1010, guidelines for studies on the new chemical substance as required by the Chemical Substance Control Law (Japan) and JMAFF 2-7-2-1 under GLP conditions. The study was assigned a reliability score of 1 in accordance with the criteria for assessing data quality set forth by Klimisch et al. (1997).
Following a preliminary range-finding test in which a toxic response was not observed, a limit test was carried out using only one test concentration (100 mg/L) and one control group in a static system. Twenty animals, divided into four replicates of five animals each were used at the test concentration and for the control. The immobility or mortality of the Daphnia was determined by visual observation 24 and 48 hours after the start of the test. The number of immobilised animals and the percentage of immobility were determined after 24 and 48 hours.
No immobility was observed either in the treatment or control vessels. In addition to immobility, no abnormal behaviour or appearance of test animals was detected.
There were no immobilised animals in the control group and the dissolved oxygen concentration at the end of the test in control and test vessels was more than 3 mg/L. All validity criteria were within acceptable limits and therefore the study can be considered as valid.
The test concentration was analytically determined at the start and at the end of the experiment. Measured concentration was 106 mg/L at the start and 103 mg/L at the end of the experiment. As the measured concentrations deviated not more than 20 per cent from the nominal, biological results are based on the nominal concentration.
Under the conditions of this study, the 48 h EC50 was >100 mg/L and the 48 h NOEC was 100 mg/L.
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