2,2-bis(allyloxymethyl)butan-1-ol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Recent study following OECD guidelines under GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Remarks:

Claimed but the GLP statement mentioned in the index is not attached to the report.

Test material

Test material information

Constituent 1

Reference substance name:

2,2-bis(allyloxymethyl)butan-1-ol

EC Number:

211-661-1

EC Name:

2,2-bis(allyloxymethyl)butan-1-ol

Cas Number:

682-09-7

Molecular formula:

C12H22O3

IUPAC Name:

2,2-bis[(prop-2-en-1-yloxy)methyl]butan-1-ol

Details on test material:

Trimethylolpropane diallyl ether 80 (P950128)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not relevant

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

Test solutions were 250 mL glass E-bottles with 100 mL solution that were incubated on a shaking table with approximately 100 µEm-2s-1 continuous illumination at a temperature of 24 ±1⁰C.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

The inoculum was taken from Toxicon's culture which originates from Swedish Environmental Protection Agency, Stockholm, Sweden.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

No post observation period.

Test conditions

Hardness:

Not stated

Test temperature:

24.5 - 25.0⁰C

pH:

8.3 - 8.7 at the start and end of the exposure period for the control, 8.3 - 8.5 for the 100 mg/L treatment.

Dissolved oxygen:

Not applicable

Salinity:

Not applicable

Nominal and measured concentrations:

A single concentration of 100 mg/L

Details on test conditions:

The test solutions were incubated on a shaking table with approximately 100 µEm-2s-1 continuous illumination at a temperature of 24 ±1 ⁰C. The media and the nutrient concentration was the same in all test solutions and the control. The incubation period was 72 hours with sampling at 0, 24, 48 and 72 hours.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

After 72 hours the mean control fluorescense was 46.3 (5 replicates, range 38.3 - 51.0). The mean value in the 100 mg/L treatment was 36.9 (2 replicates, range 34.8 - 39.0, one replicate was deemed to be an outlier and not used 2.7) The mean growth rate in the control was 1.53 and in the 100 mg/L treatment was 1.40.

The % inhibition in the 100 mg/L treatment was 8.1%, therefore the 72 h EC50 (based on nominal exposure concentrations) was >100 mg/L.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

The result was based on observation of the data.

Any other information on results incl. tables

The concentration tested (100 mg/L) gave only a slight inhibition of the growth rate ie. the compound is not considered as acute toxic to the green algae, Selenastrum capricornutum. The results table is shown below;

Conc mg/L	pH		Fluorescence (chlorophyll a)					Growth Rate, µ	% inhibition
	0 h	72 h	0 h	24 h	48 h	72 h (dil 3 times)	72 h x 3
Control 1	8.3	8.7	2.3	2.7	15.1	17.0	51.0
Control 2			2.0	3.0	14.2	16.6	49.8
Control 3			2.0	3.8	13.0	16.0	48.0
Control 4			2.1	3.6	11.7	14.8	44.4
Control 5			1.3	2.8	10.8	12.8	38.4
mean			1.9	3.2	13.0		46.3	1.53
100	8.3	8.5	2.1	2.5	3.3	-	2.7*
100			2.0	3.3	11.4	13.0	39.0
100			1.9	3.3	10.3	11.6	34.8
mean			2.0	3.0	8.3		36.9	1.40	8.1

* - valued determined to be an outlier and not used in the calculation of the results

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The concentration tested (100 mg/L) gave only a slight inhibition of the growth rate ie. the compound is not considered as acute toxic to the green algae, Selenastrum capricornutum.

Executive summary:

The toxicity of TMPDE 80 to the green algae,Selenastrum capricornutum, was determined in the 72 hour test conducted according to OECD 201. The algae were exposed to a single concentration of TMPDE 80 of 100 mg/L. The 72hour EC₅₀(based on growth rate) was observed to be >100 mg/L (based on nominal exposure concentrations). The corresponding NOEC was 100 mg/L.