2,2-bis(allyloxymethyl)butan-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Recent study performed under GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2-bis(allyloxymethyl)butan-1-ol

EC Number:

211-661-1

EC Name:

2,2-bis(allyloxymethyl)butan-1-ol

Cas Number:

682-09-7

Molecular formula:

C12H22O3

IUPAC Name:

2,2-bis[(prop-2-en-1-yloxy)methyl]butan-1-ol

Details on test material:

The test item is registered under the trade name, trimethylolpropane diallyl ether 90 (TMPDE 90). The actual composition of the test item is trimethylolpropane diallyl ether, 90.7%, trimethylolpropane monoallyl ether, 5.2 % and trimethylolpropane triallyl ether, 3.9%.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No further details

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

5006 mg of the test item was dissolved in 1000 mL of test water (dilution water) by magnetic stirring on the day of the test start. The stock solution (approx 5000 mg/l) was diluted with reconstituted test water in order to prepare the test solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organisms are 6 to 24 hours old, which are incubated for48 hours in a gradient of the test item.The animals were taken from Toxicon's continuous culture of Daphnia magna. The culture originates from NIVA, Norway and is on working days fed with approximately 50-75 mL green algae Pseudokirchneriella subcapitata.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure observation period.

Test conditions

Hardness:

250±25 mg/l

Test temperature:

21±1 ⁰C

pH:

The pH was measured at the start and the end of the experiment. pH at the start ranged between 7.66 and 7.99, and between 8.04 and 8.07 at the end of the exposure period.

Dissolved oxygen:

The dissolved oxygen (DO) concentration was measured at the start and the end of the experiment. DO at the start ranged between 96.2 and 97.6%, and between 99.4 and 100.6% at the end of the exposure period.

Salinity:

Not stated.

Nominal and measured concentrations:

The nominal concentrations of the test item in the test solutions were 25, 50, 100, 200 and 400 mg/L.

Details on test conditions:

The animals were incubated in glass Petri dishes (50 mL) with 25 mL solution. Four replicates with five animals in each replicate were used for the both the test concentrations and for the control. The dishes were incubated in a thermo constant room at temperature 21±1 ⁰C and subdued light with the light rhythmof 16 hours light: 8 hours darkness.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

189 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

An EC50 value of 189 mg/L was determined for the 48 hours of exposure for the test item TMPDE 90.

Results with reference substance (positive control):

A qualification test on the Daphnia magna culture was made on 15 May 2008 to assure the quality of the test animals. EC50 was 1.1 mg/L, which confirmed that the culture was suitable as a test organism.

Reported statistics and error estimates:

No reported stastics and error estimates.

Any other information on results incl. tables

Immobility after 24 hours exposure in the control, 25, 50, 100, 200 and 400 mg/L treatments was 0, 5, 0, 10,40 and 25%.

Immobility after 48 hours exposure in the control, 25, 50, 100, 200 and 400 mg/L treatments was 0, 5, 0, 10, 55 and 100%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of TMPDE 90 to the water flea, Daphnia magna, was determined in the 48 hour acute immobilisation test conducted according to OECD 202. The daphnids were exposed to concentrations of TMPDE 80 between 25 and 400 mg/L. The 48 hour EC50 was calculated to be 189 mg/L (based on nominal exposure concentrations). The corresponding NOEC was 100 mg/L.

Executive summary:

The toxicity of TMPDE 90 to the water flea, Daphnia magna, was determined in the 48 hour acute immobilisation test conducted according to OECD 202. The daphnids were exposed to concentrations of TMPDE 90 between 25 and 400 mg/L. The 48 hour EC₅₀was calculated to be 189 mg/L (based on nominal exposure concentrations). The corresponding NOEC was 100 mg/L.