Tetrabutylammonium hydrogen sulphate

Endpoint summary

Description of key information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium hydrogen sulphate The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L

As no mortality was observed at highest loading thus LC0 (96 hours) was consider to be 100 mg/L.

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted forN,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no 32503-27-8). EC50 value was estimated to be 156.38mg/L on the basis of mobility for Daphnia magna for 48 hrs duration. Based on this value it can be concluded that theN,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no 32503-27-8)was considered to be not classified to aquatic invertebrates as per the CLP regulations.

 

Toxicity to aquatic algae and cyanobacteria

The effect of test item Tetrabutylammonium hydrogen sulphate, CAS No. 32503-27-8 was studied on the growth of fresh water green alga Chlorella vulgaris(UERL Study report, Sustainability Support Services (Europe) AB, Report no. 32503-27-8/01/2017/AT, 2017).The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 19.804 mg/L. Thus based on this value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP classification criteria.

Additional information

Short-term toxicity to fish:

The test chemical N,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no. 32503-27-8) and its relevant read across which is 80-90% structurally similar were reviewed to summarise the following information: 

An experiment was conducted by UERL (2017) to determine the toxic effects of the test compound chemical Tetrabutylammonium hydrogen sulphate (Cas no. 32503-27-8) on the ZEBRA FISH (Danio rerio).

According to study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Tetrabutylammonium hydrogen sulphate.The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.

As no mortality was observed at highest loading thus LC0 (96 hours) was consider to be 100 mg/L.

Whereas the 96 hrs aquatic toxicity effect was predicted to assess toxic effects of the test compound N,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no. 32503-27-8) (QSAR toolbox ver.3.4, 2017). The study was based on the effects of the test compound on fish Oncorhynchus mykiss (previous name: Salmo gairdneri) in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound was estimated to be 162.48 mg/l on the basis of mortality effect. Thus, based on this value, it can be concluded that the test chemical N,N,N-tributylbutan-1-aminium hydrogen sulfate can be considered as non toxic to fish and thus not consider for aquatic classification as per the CLP regulation.

Another prediction model ECOSAR v1.1; (2017) from EPI suite for target chemical N,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no. 32503-27-8) indicates that the lethal concentration (LC50) for the N,N,N-tributylbutan-1-aminium hydrogen sulfate(CAS no 32503-27-8) was estimated to be 2389.214 mg/L. Hence, N,N,N-tributylbutan-1-aminium hydrogen sulfate(CAS no 32503-27-8) was not considered being hazardous to fish and can be considered to be not classified as per the CLP regulation.

In addition, supporting experimental data (UERL laboratory result; 2013) for read across N,N,N-tributylbutan-1-aminium bromide (Cas no. 1643-19-2) indicated that 96 h study for tetrabutylammonium bromide on Poecilia reticulata, at a test concentration of 100 mg/L was conducted. The fishes were found to be lethargic yet swimming near the water surface. No mortalities were observed on 4 day (96 h) in the test concentration 100 mg/l. No mortalities were found in the control aquaria. Hence the LC50 was considered to be > 100 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that tetrabutylammonium bromide does not exhibit short term toxicity to fish. 

Thus, the study of read across chemical aupports the remaining study results of target. Hence, based on above studies, it can be concluded that the target chemical, N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no. 32503-27-8) can be considered as non toxic to fish and thus not consider for aquatic classification as per the CLP regulation criteria.

Short term toxicity to aquatic invertebrates:

Short term toxicity to aquatic invertebrates was reviewed for N, N, N-tributylbutan-1-aminium hydrogen sulfate (CAS no 32503-27-8) with two predicted data and one experimental report for read across tetrabutylammonium bromide (1643-19-2) are as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for N, N, N-tributylbutan-1-aminium hydrogen sulfate (CAS no 32503-27-8). EC50 value was estimated to be 156.38mg/L on the basis of mobility for Daphnia magna for 48 hrs duration.

Another prediction on short-term toxicity of the substance to invertebrate was done using EPI suite, ECOSAR version 1.1, 2017, based on the effects observed in 48 hr exposure. The lethal concentration (LC50) for the substance is estimated to be 1232.557 mg/L.

For the read across substance tetrabutylammonium bromide (CAS: 1643-19-2) by C. Pantani, et. al. (1995), a short term toxicity test (24 h) was undertaken to evaluate the effect of tetrabutylammonium bromide (TBABr) to Echinogammarus tibaldii, a benthic shredder amphipod with an important role in the detritus chain of rivers and streams. Organisms of the species E.tibaldii Pink & Stock (Crustacea, Amphipoda) were collected near the spring of the river Vera. The test was conducted following the guideline of OECD. The definitive tests were carried out in duplicate by adding 20 animals (Mature adult male) in each glass jar containing 250 mL of each precooled and aerated solution.The test carried out in static conditions without oxygenation; the animals were also not fed during experiment. After the experimental period the LC 50 value for tetrabutylammonium bromide (TBABr) (1643 -19 -2) for short term toxicity to aquatic invertebrates was determined to be >2000 mg/l.

On the basis of predicted results for N,N,N-tributylbutan-1-aminium hydrogen sulfate(CAS no 32503-27-8) and its read across, it can be considered that all the above weight of evidences has value greater than 100 mg/l which indicate that N,N,N-tributylbutan-1-aminium hydrogen sulfate (CAS no 32503-27-8) was not likely to be toxic to aquatic invertebrates at environmentally relevant concentrations and can be considered to be not classified as per the criteria of CLP regulation.

Toxicity to aquatic algae and cyanobacteria

The effect of test item Tetrabutylammonium hydrogen sulphate, CAS No. 32503-27-8 was studied on the growth of fresh water green alga Chlorella vulgaris(UERL Study report, Sustainability Support Services (Europe) AB, Report no. 32503-27-8/01/2017/AT, 2017).The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 3.125 mg/L, 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L,100 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 19.804 mg/L. Thus based on this value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP classification criteria.