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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 July 2011 to 30 July 2011
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OSPAR Commission 2006 Part B. Protocol for a fish acute toxicity test Study number 1531a-22 was commissioned by Nalco Limited to determine the aquatic (96h limit test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- other: Solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Samples not taken
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Natural local seawater is pumped to holding tanks from Scapa Flow, Orkney. The treatment process was: sand filtered to remove particulate, filtered to 1μm, heated to 16 ºC, UV treated, degassed and held at a quantity of 3000 to 5000 litres. The water is constantly pumped through the system and trickle replenished as the water is used.
In order to determine an appropriate test preparation method, an assessment was made of its behaviour in seawater. A 1000 mg.l-1 stock was prepared in filtered seawater, and the resulting mixture was stirred for one hour. If the material was observed to be soluble then its behaviour was assessed. If it was poorly soluble after one hour stirring then it was stirred for approximately a further 19 hrs, and then left to settle for one hour and its behaviour assessed, and prepared according to SOP 403. Readily soluble materials were added directly via treated seawater to the appropriate tank in the required quantity.
The test substance was characterised as poorly soluble and therefore prepared as Water Accommodated Fraction (WAF). Solutions of 2 litres were prepared, stirred for 20 hours and then one litre siphoned into the test vessels.
Test organisms
- Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- The test organisms were reared and maintained at Opus Plus Ltd. The length of fish was 1.5-2.5 cm and at test termination control fish were measured to confirm the length range. Sheepshead minnow were held under flow-through conditions in 50 litre capacity glass tanks, containing 1 μm filtered ultra-violet treated seawater and supplied with gentle aeration. Water quality measurements and mortality observations were conducted daily and fed food in flake form ad libitum at least twice daily. Fish were randomly selected for testing.
Study design
- Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- Temperature was measured at each concentration prior to and following each renewal of media.
The temperature range during the test was 18.5 - 20.7ºC - pH:
- pH was measured at each concentration prior to and following each renewal of media.
the pH range over 96h was 7.94 - 8.04 - Dissolved oxygen:
- Dissolved oxygen was measured at each concentration prior to and following each renewal of media.
Dissolved oxygen was 93 - 98% during the test - Salinity:
- Salinity was measured at each concentration prior to and following each renewal of media.
Salinity was 36 - 38% during the test - Nominal and measured concentrations:
- Nominal test concentration 65.06 mg/l
- Details on test conditions:
- The tests were conducted in a temperature controlled room to maintain 20±2 ºC in the test medium. The tests were conducted in 15 litre capacity glass tanks containing 3 litres of test media. The preparations were mixed in situ to ensure thorough dissolution before introduction of the test organisms. Gentle aeration was supplied to all tanks.
Limit tests were conducted under semi-static conditions (with replacement of media after 48h) over 96h with ten fish exposed to the single concentration along with one control.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 65.06 other: mg/L WAF
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The test substance exhibited no effect at 65.06mg.l-1 after 96h (Water Accommodated Fraction (WAF)) to the marine fish Cyprinodon variegatus in the water phase.
- Reported statistics and error estimates:
- Mortalities were determined in each vessel every 24h period. The number of dead organisms were expressed as a proportion of the total number exposed.
No statistical methods were employed in the limit test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance exhibited no effect at 65.06mg.l-1 after 96h (Water Accommodated Fraction (WAF)) to the marine fish Cyprinodon variegatus in the water phase.
- Executive summary:
Acute toxicity (96 h LC50semi-static test) of the test substance to Cyprinodon variegatus has been assessed in accordance with OSPAR Commission 2006 Part B. Protocol for a fish acute toxicity test
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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