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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: inhalation-hazard test, sufficient information to characterize the effects on animals when exposed by inhalation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
6 male Wistar rats/exposure-period were exposed to saturated vapor atmosphere (whole body exposure) for 4, 5, 6 or 8 hours, respectively. Generation of the test atmosphere was carried out at room temperature (24-25 °C) by a stream of air being bubbled through the testsubstance. Concentration of the test atmosphere was determined. Animals were carefully observed during exposure and up to 14 days post exposure with feed and water ad libitum. After termination of the test animals were sacrificed and gross pathologically examined.
GLP compliance:
no
Test type:
other: inhalation-hazard test

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chlorotoluene
EC Number:
203-397-0
EC Name:
4-chlorotoluene
Cas Number:
106-43-4
Molecular formula:
C7H7Cl
IUPAC Name:
4-chlorotoluene
Details on test material:
p-chlorotoluene (no data on purity)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
>= 4 - <= 8 h
Concentrations:
4182.6, 4102.4, 3950.9, or 4304.2 ppm (ca. 22000.4, 21578.6, 20781.7, 22640.0 mg/m³)
No. of animals per sex per dose:
6
Control animals:
not specified

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 21.5 mg/L air
Exp. duration:
4

Any other information on results incl. tables

Signs of intoxication:
disturbed balance, slightly closed eye lids, lowered reflex
answer to acoustic stimuli, tremor, tachypnea, hypopnea, anestesia, death occured from 5 hour-exposure test onwards

---4-hour-exposure,
test atmosphere approximately 4182.6 ppm:
No animal died during exposure; rats recovered 24 hours post exposure
gross pathology: no finding

---5-hour-exposure,
test atmosphere approximately 4103.4 ppm:
3/6 rats died within the first night post exposure; 3/6 rats survived and recovered 24 hours post exposure
gross pathology: decedents showed lungs slightly red colored; survivors: no findings

---6-hour-exposure,
test atmosphere approximately 3950.9 ppm:
3/6 rats died during exposure; 2/6 rats died within the first night post exposure; 1/6 rats died 24 hours later
gross pathology: 5/6 dead rats had red colored lungs

---8-hour-exposure,
test atmosphere approximately 4304.2 ppm:
6/6 rats died within the first night post exposure
gross pathology: all rats had marked red lungs

Applicant's summary and conclusion

Interpretation of results:
moderately toxic
Remarks:
Migrated information
Executive summary:

In an inhalation hazard test male rats were exposed to 4182.6, 4102.4, 3950.9, or 4304.2 ppm (ca. 22000.4, 21578.6, 20781.7, 22640.0 mg/m³) for 4, 5, 6, or 8 hours.

result: rats exposed for 5 hours to a concentration of 4103.4 ppm (ca. 21.5 mg/L) 3 out of 6 animals died. Therefore the LC50 = ca. 21.5 mg/L.