Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-05-14 to 1991-05-31
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD and GLP study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Each 24hrs new testing basins were prepared
- Using 0,095mL of Tween80/L and the testing compound a paste was prepared
- To this paste dilution water was added
- Both was homogenised using ultra-turrax and ultrasonic bath
- Stirring constantly the homogenate was transferred to the testing basin
- There, the whole basin was stirred for approx. 2hrs using a KPG-stirrer and a glass stirrer
- The test solution was clear with some sedimation of substance on the ground of the basin
- Analytical determination was +/- 20% of the expected concentration

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
Test solution: 21,4 - 21,9°C
Control: 21,4 - 22,3°C
pH:
Test solution: 7,9 - 8,3
Control: 7,9 - 8,3
Dissolved oxygen:
Test solution: 6,9 - 8,9 mg/L
Control: 7,6 - 9,4 mg/L
Nominal and measured concentrations:
1) nominal: 100mg/L, measured: 105,5mg/L
2) nominal: 100mg/L, measured: 100,5mg/L
3) nominal: 220mg/L, measured: 226 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
148.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
220 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: In all tested concentrations the swimming behavior and breathing was affected as well as modified appearance and behavior was observed.

- Dead fish showed a dark discoloration, red gills, lockjaw and the body was bend.

Any other information on results incl. tables

Table 1:

Concentration

(mg/l)

Mortality

48 h

96 h

absolute

relative (%)

absolute

relative (%)

0

0/20

0

0/20

0

0*

0/10

0

0/10

0

100

0/10

0

0/10

0

220

10/10

100

10/10

100

LC0 (mg/l)

highest tested concentration without deaths

100

 

100

 

LC50 (mg/l)

148.3

 

148.3

 

LC100 (mg/l)

lowest tested concentration with 100 % deaths

220

 

220

 

0* = control group with 0.095 ml/l emulsifying agent but no test substance

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
not subject of classification. LC0 = 100 mg/l