Diphenylacetonitrile

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity to micro-organism studies as-
2. and 3.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Photobacterium phosphoreum

Test type:

static

Water media type:

saltwater

Total exposure duration:

30 min

Test temperature:

2. 15ᵒC
3. No data available

pH:

2. 5 to 9
3. No data available

Reference substance (positive control):

yes

Remarks:

2. Recommended reference substance: Phenol and Sodium pentachlorophenate, 3. No data available

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

4.397 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

other: Reduction in light output

Remarks on result:

other: 2.

Duration:

15 min

Dose descriptor:

EC50

Effect conc.:

4.207 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

other: Reduction in light output

Remarks on result:

other: 2.

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

4.601 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Reduction in light output

Remarks on result:

other: 2.

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 3.

2.

Table: Toxicity data

 

#	5 m- pT	15 m- pT	30 m- pT	Name	Formula	ppm	M. wt.
164	1.49	1.51	1.47	Test chemical	C8H8O2	4.61	136.15

 

Where,

Footnote = Purity of chemical (in %) and/or deviation from standard conditions.

pT =Measured concentrations (in log(L/mmol) at which there is a 50% reduction of the light emission relative to controls at the stated exposure times of 5, 15 and 30 min.

ppm =The compound's toxicity expressed in parts per million (ppm) or mg/L. If more than one entry is provided on the same line under the pT headings, preference is given to the 30-min exposure EC50 level, second preference to the 15-min level and third preference to the 5-min level.

3. No data available

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on the luminescence of the test organism Photobacterium phosphoreum can be expected to be 1.3 mg/l and 4.6 mg/l, respectively.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on micro-organisms.The studies are as mentioned below:

Aquatic toxicity study of micro-organism to the test chemical was conducted using Photobacterium phosphoreum, strain NRRL-B-11177 (also referred to asVibrio fischerii, strain NRRL-B-11177). The study was performed at a temperature of 15ᵒC and pH range 5 to 9, respectively. Recommneded reference substance that can be used for the study were Phenol and Sodium pentachlorophenate, respectively. When the test bacterium Photobacterium phosphoreum, strain NRRL-B-11177 was exposed to the test chemical, reduction in light output was observed. Thus, based on this effect, the EC50 value during 5, 15 and 30 min exposure period was determined to be 4.39, 4.2 and 4.6 mg/l, respectively.

For the test chemical, toxicity to microorganism study was carried out for 30 mins. Photobacterium phosphoreum was used as a test organism. On the basis of the effect of test chemical on growth rate, i.e, cell multiplication of the test organism Photobacterium phosphoreum, the EC50 value was determined to be 1.3 mg/l.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on the luminescence of the test organism Photobacterium phosphoreum can be expected to be 1.3 mg/l and 4.6 mg/l, respectively.

Description of key information

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on the luminescence of the test organism Photobacterium phosphoreum can be expected to be 1.3 mg/l and 4.6 mg/l, respectively.

Additional information

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on micro-organisms. The studies are as mentioned below:

 

Aquatic toxicity study of micro-organism to the test chemical was conducted using Photobacterium phosphoreum, strain NRRL-B-11177 (also referred to as Vibrio fischerii, strain NRRL-B-11177). The study was performed at a temperature of 15ᵒC and pH range 5 to 9, respectively. Recommneded reference substance that can be used for the study were Phenol and Sodium pentachlorophenate, respectively. When the test bacterium Photobacterium phosphoreum, strain NRRL-B-11177 was exposed to the test chemical, reduction in light output was observed. Thus, based on this effect, the EC50 value during 5, 15 and 30 min exposure period was determined to be 4.39, 4.2 and 4.6 mg/l, respectively.

 

For the test chemical, toxicity to microorganism study was carried out for 30 mins. Photobacterium phosphoreum was used as a test organism. On the basis of the effect of test chemical on growth rate, i.e, cell multiplication of the test organism Photobacterium phosphoreum, the EC50 value was determined to be 1.3 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on the luminescence of the test organism Photobacterium phosphoreum can be expected to be 1.3 mg/l and 4.6 mg/l, respectively.