2,3,6-trimethylphenol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report which meets basic scientific principles; pre-GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

reading after 1,24hours, 8 days, 2 animals

Principles of method if other than guideline:

BASF-Test

GLP compliance:

no

Remarks:

pre-GLP study

Test material

Test material information

Constituent 1

Reference substance name:

2,3,6-trimethylphenol

EC Number:

219-330-3

EC Name:

2,3,6-trimethylphenol

Cas Number:

2416-94-6

Molecular formula:

C9H12O

IUPAC Name:

2,3,6-trimethylphenol

Details on test material:

- Name of test material (as cited in study report): 2,3,6-trimethyl-phenol (TMP)
No further data

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

dark/light rhythm 12/12 hours

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: talcum

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 spoon of the kristalline unchanged substance

Duration of treatment / exposure:

up to 8 days, no substance wash out

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no

SCORING SYSTEM:
BASF scores, convertible into Draize scores, readings at 24 hours and 8 days after beginning of application

TOOL USED TO ASSESS SCORE: no data

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 0-8 days

Score:

>= 1 - <= 3

Max. score:

3

Reversibility:

not reversible

Remarks on result:

other: staphyloma

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 0-8 days

Score:

>= 0 - <= 1

Max. score:

1

Reversibility:

fully reversible

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 0-8 days

Score:

>= 1 - <= 2

Max. score:

2

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: staphyloma

Irritant / corrosive response data:

see table

Any other information on results incl. tables

Draize scores and findings:

Reading	Animal no	Treated eye					Control eye
		Cornea	Iris	Redness	Edema	Remarks	Redness	Remarks
1 hour	1	1	0	1	3-4	-	2	RS
	2	1	0	1	2	-	2	RS
24 hours	1	3	1	2	2	H	2
	2	3	1	2	2	-	0
8 days	1	3	0	2	0	S, IV	0
	2	3	0	0	0	IV	0

Legend: abbreviations used for remarks

RS = residues of the test substance

H = hemorrhages

S = staphyloma

IV = ingrowing vessels

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Remarks:

Migrated information Criteria used for interpretation of results: EU

Executive summary:

The test substance caused severe damage to the eyes when instilled unchanged into the conjunctival sac of the eye of the rabbits.