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EC number: 202-039-0 | CAS number: 91-08-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Chemical behavior of seven aromatic diisocyanates (toluenediisocyanates and diphenyl methane diisocyanates/ under in vitro conditions in relationship to their results in the Salmonella/microsome test
- Author:
- K. Seel , U. Walber a, B. Herbold b, R. Kopp
- Year:
- 1 999
- Bibliographic source:
- Mutation Research 438, 109–123
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Similar to OECD 471
- Principles of method if other than guideline:
- Salmonella/microsome test was performed on 2, 6 Toluene diisocyanate chemical to know its mutagenic effect.
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- 2-methyl-m-phenylene diisocyanate
- EC Number:
- 202-039-0
- EC Name:
- 2-methyl-m-phenylene diisocyanate
- Cas Number:
- 91-08-7
- Molecular formula:
- C9H6N2O2
- IUPAC Name:
- 1,3-diisocyanato-2-methylbenzene
- Details on test material:
- - Name of test material: 2,6 Toluene diisocyanate
- Molecular formula: C9H6N2O2
- Molecular weight: 174.1584 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data available
- Impurities (identity and concentrations): No data available
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: Salmonella typhimurium LT2 mutants TA 1535, TA 100, TA 1537 and TA 98
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction was obtained from Aroclor 1254 500 mgrkg in corn oil, single intraperitoneal injection, 5 days prior to sacrifice. induced male Sprague–Daw- ley rats
- Test concentrations with justification for top dose:
- 0,150,300,600,1200,2400 and 4800µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethyleneglycoldimethylether (EGDE)
- Justification for choice of solvent/vehicle: No data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene with S9 metabolic activation system
- Remarks:
- No data on control chemical available
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: No data available
- Exposure duration: 48 hrs
- Expression time (cells in growth medium): 48 hrs
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: Duplicate. Three plates were used for each concentration and each strain.
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY The toxicity of the substance was assessed as a gross appraisal of background growth on mutant plates and/or as a marked and dose-dependent reduction in the mutant count.
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other:
OTHER: No data available - Evaluation criteria:
- A reproducible and dose-related increase in mutant counts of at least one strain is considered to be a positive result. For TA 98, this increase should be about twice that of negative controls, whereas for TA 1537 at least a threefold increase is required.
- Statistics:
- No data available.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA 1535, TA 100, TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Remarks:
- upto 150 µg/plate for 10% S9 mix and upto 300 µg/plate for 30% S9 mix
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data
- Effects of osmolality: No data
- Evaporation from medium: No data
- Water solubility: No data
- Precipitation: Yes, observed
- Other confounding effects: No data
RANGE-FINDING/SCREENING STUDIES: No data
COMPARISON WITH HISTORICAL CONTROL DATA: No data
ADDITIONAL INFORMATION ON CYTOTOXICITY: No data - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table: Result of 2,6 TDI dissolved in EDGE and metabolic activation
Strain S9, µg/plate |
TA1537 |
TA98 |
||
10% S9 |
30% S9 |
10% S9 |
30% S9 |
|
0 |
12 |
14 |
47 |
45 |
150 |
13 |
21 |
67 |
57 |
300 |
13 |
16 |
87* |
61 |
600 |
15 |
15p |
130* |
73* |
1200 |
7p |
10p |
166*p |
97*p |
2400 |
7p |
9p |
128*p |
82*p |
4800 |
P |
P |
P |
P |
2-Aminianthracene |
360* |
66* |
1538* |
618* |
*: Mutagenic effect
p: precipitation
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative With and without Salmonella typhimurium TA 1535, TA 100, TA 1537
negative without metabolic activation Salmonella typhimurium strain TA98
negative with metabolic activation Salmonella typhimurium strain TA98; upto 150 µg/plate for 10% S9 mix and upto 300 µg/plate for 30% S9 mix
2, 6 Toluene diisocyanate is negative for the strains TA 1535, TA1537 and TA100 with and without S9 activation system and negative for Salmonella typhimurium strain TA98 upto 150 µg/plate for 10% S9 mix and upto 300 µg/plate for 30% S9 mix. Hence 2, 6 Toluene diisocyanate is not likely to classify as a gene mutant in vitro. - Executive summary:
Salmonella/microsome test was performed on 2, 6 Toluene diisocyanate to determine its mutagenic effect in the bacterial tester strains TA1535, TA1537, TA100 and TA98 of Salmonella typhimurium LT2 mutants. Plate incorporation method was performed and the reversion count was made after 48hrs incubation period.
2, 6 Toluene diisocyanate is negative for the strains TA 1535, TA1537 and TA100 with and without S9 activation system and negative for Salmonella typhimurium strain TA98 upto 150 µg/plate for 10% S9 mix and upto 300 µg/plate for 30% S9 mix. Hence 2, 6 Toluene diisocyanate is not likely to classify as a gene mutant in vitro.
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