2,6-dimethyloct-7-en-2-yl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

TA102 strain is missing

Justification for type of information:

In addition to the Ames test on Dihydromyrcenyl acetate, Ames information from Dihdyromyrcenol is added to make up for the missing TA102 strain. The read across rationale is presented in the Genetic toxicity Endpoint summary, the accompanying files are also attached there.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Ames test method et al. (1975)

Version / remarks:

Ames test method et al. (1975)

GLP compliance:

no

Remarks:

conduected prior to the GLP guideline

Type of assay:

bacterial forward mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,6-dimethyloct-7-en-2-yl acetate

EC Number:

258-751-7

EC Name:

2,6-dimethyloct-7-en-2-yl acetate

Cas Number:

53767-93-4

Molecular formula:

C12H22O2

IUPAC Name:

2,6-dimethyloct-7-en-2-yl acetate

Test material form:

liquid

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 98

Metabolic activation:

with and without

Test concentrations with justification for top dose:

0, 0.008, 0.04, 0.2, 1.0 and 5.0 microL/plate

Vehicle / solvent:

Appropriate dilutions in DMSO were prepared immediately before use.

Controls

Untreated negative controls:

yes

Positive controls:

yes

Positive control substance:

not specified

Details on test system and experimental conditions:

2 ml molten soft agar were added to 0.1 mL of a fully grown culture of one of the tester strains and 0.1 mL of the appropriate dilution /suspension of the test compound (and 0.5 mL of the S-9 mix if indicated). The ingredients were thoroughly mixed and immediately poured into minimal glucose agar plates. After the top agar had been allowed to harden, the plates were incubated at 37°C for two days. Then the colonies (revertants which are histidine-independant) were counted, and the background lawn of baterial growth examined microscopically. All determination were made in triplicate and appropriate controls were included in each assay.

Results and discussion

Test results

Key result

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at 1.0 and 5.0 microL/plate DHMA was clearly toxic to strains TA1537, TA1538, TA98 and TA100 in the absence of S9

Vehicle controls validity:

not valid

Positive controls validity:

valid

Additional information on results:

Incorporation of 0.008 up to 0.5 - 0.5 microL of the test liquid par plate did not induce an increase in the number of his +revertants with any of the five tester strains either in the presence ot absence of S-9 mix. At 1.0 and 5.0 microL/plate DHMA was clearly toxic to strains TA1537, TA1538, TA98 and TA100 in the basence of S9 mix as revealed by a less dense background lawn of bacterial growth.

Applicant's summary and conclusion

Conclusions:

From the present results it can be concluded that the substance up to non- inhibitory levels did not reveal any mutagenic activity in the plate incorporation assay with S.Typhimurium TA1535, TA1537, TA1538, TA98 or TA100 in the presence or absence of the liver microsome activation system under the test condition employed in this evaluation.

Executive summary:

The mutagenic activity of the substance was examined in the Salmonella/microsome mutagenicity test, using a set of five histidine requiring mutants of S. Typhimurium (TA1535, TA1537, TA1538, TA98 and TA100) and liver homogenate of Aroclor induced rats. Incorporation of the test liquid up to non-inhibitory levels i.e. 0.2 - 5.0 microl/plate did not increase the number of his +revertants in any of the five tester strains either in the presence or in the absence of the liver microsome activation system.

It was concluded that the present results did not reveal any mutagenic activity of the test material in the Salmonella/microsome metagenicity test.