Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Experimental test result performed using standard test guideline
Justification for type of information:
Experimental test result performed using standard test guideline

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Aim of this study was to evaluate the nature of chemical when comes in contact with the test organism. Test was conducted according to the OECD guideline 201.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
dialuminium(3+) tris(5-amino-4-hydroxy-3-[(1E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate)
Constituent 2
Reference substance name:
Test material form:
other: Solid
Details on test material:
- Name of test material (as cited in study report): Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium- Molecular formula (if other than submission substance): C48H33Al2N9O21S6- Molecular weight (if other than submission substance): 1318.19 g/mol- SMILES:c12c(cc(c(c2O)\N=N\c2ccccc2)S([O-])(=O)=O)cc(S(=O)(=O)[O-])cc1N.[Al+3].[Al+3].c12c(cc(cc2cc(c(c1O)\N=N\c1ccccc1)S(=O)(=O)[O-])S(=O)(=O)[O-])N.c12c(cc(cc2cc(c(c1O)\N=N\c1ccccc1)S(=O)(=O)[O-])S(=O)(=O)[O-])N- InChI: 1S/3C16H13N3O7S2.2Al/c3*17-12-8-11(27(21,22)23)6-9-7-13(28(24,25)26)15(16(20)14(9)12)19-18-10-4-2-1-3-5-10;;/h3*1-8,20H,17H2,(H,21,22,23)(H,24,25,26);;/q;;;2*+3/p-6/b3*19-18+;;- Substance type: Organic- Physical state: Solid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

not specified
Details on test solutions:
The stock solution 322.5 mg/L was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM- Common name: - Strain: 86.81 SAG- Source (laboratory, culture collection): Institute of botany of the ASCR- Initial biomass concentration: 5x10(3) cells /ml - Method of cultivation: No data availableACCLIMATION - No data available- Acclimation period:- Culturing media and conditions (same as test or not):- Any deformed or abnormal cells observed:

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
72 h

Test conditions

Test temperature:
Nominal and measured concentrations:
10,20,40,80,160 mg/L
Details on test conditions:
TEST SYSTEM- Test vessel:150 ml Glass vessel- Type (delete if not applicable): closed (with air permeable stopper)- Sample volume: -------- ml- Initial cells density: 5x10(3) cells/ml- No. of vessels per concentration (replicates): --------GROWTH MEDIUM- Standard medium used: yesOTHER TEST CONDITIONS- Adjustment of pH: No- Photoperiod: Continuous- Light intensity and quality: 6000-8000 lxEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
Potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations
Key result
72 h
Dose descriptor:
Effect conc.:
322.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CI
Results with reference substance (positive control):
- Results with reference substance valid- EC50: ----- mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0 OR (The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05.)

Applicant's summary and conclusion

Validity criteria fulfilled:
The median effective concentration (ErC50) for the test substance,Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Executive summary:

Aim of this study was to assess the short term toxicity ofTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminiumto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.


The stock solution 160 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).-10,20,40,80,160 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.


The median effective concentration (EC50) for the test substanceTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium, in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.