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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Toxicity of Rhodamine B and Fluorescein Sodium to Fish and their Compatibility with Antimycin A.
Author:
Leif L. Marking
Year:
1969
Bibliographic source:
The Progressive Fish – Culturist, Vol. 31 (3), Pg. no. 139-142, 1969.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Short term toxicity study to Rainbow trout (Salmo gairdneri), Channel catfish (Ictalurus punctatus) and Bluegill (Lepomis macrochirus) was carried out for 96 hrs.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: Orange reddish powder
Details on test material:
- Name of test material (as cited in study report): Fluorescein sodium - Molecular formula (if other than submission substance): C20-H10-O5.2Na- Molecular weight (if other than submission substance): 376.274 g/mol- Smiles notation (if other than submission substance): c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]- InChl (if other than submission substance): 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2- Substance type: Organic - Physical state: Solid

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
- Concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.- Sampling method: The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.The dye for the toxicity study was weighed separately for each vessel.Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
other: 3 different test organisms were used for the study. The test organism include- 1.Rainbow trout (Salmo gairdneri) 2.Channel catfish (Ictalurus punctatus) and 3.Bluegill (Lepomis macrochirus)
Details on test organisms:
No data available

Study design

Test type:
not specified
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
LC50 value was determined at 24, 48 and 96 hrs.

Test conditions

Hardness:
No data available
Test temperature:
12°C
pH:
No data available
Dissolved oxygen:
No data available
Salinity:
No data available
Nominal and measured concentrations:
Nominal concentrations
Details on test conditions:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): LC50 value was determined at 24, 48 and 96 hrs.TEST CONCENTRATIONS- Test concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
4 198 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
3 420 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 372 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
3 828 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
2 826 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 267 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
4 898 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3 433 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
Reported statistics and error estimates:
No data available

Any other information on results incl. tables

Table: Toxicity of fluorescein sodium to test fishes at 12°C.

 

Dye

Species

LC50 and 95% confidence interval (ppm) at:

24 hrs

48 hrs

96 hrs

 

Fluorescein sodium

Rainbow trout

 

 

Channel catfish

 

 

Bluegill

4,198

(3,695 – 4,763)

 

3,828

(3,560 – 4,115)

 

5,000

(4,308 – 5,800)

3,420

(3,000 -3,900)

 

2,826

(2,559 – 3,123)

 

4,898

(4,153 – 5,765)

1,372

(1,023 – 1,840)

 

2,267

(1,928 – 2,670)

 

3,433

(2,922 – 4,028)

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-Rainbow trout: LC50 values at24 hrs - 4198 mg/l48 hrs - 3420 mg/l96 hrs –1372 mg/lChannel catfish: LC50 values at24 hrs -3828 mg/l48 hrs -2826 mg/l96 hrs –2267 mg/lBluegill: LC50 values at24 hrs -5000 mg/l48 hrs -4898 mg/l96 hrs –3433 mg/l
Executive summary:

Short term toxicity study to 3 different species of fish was carried out for 96 hrs.The test organism include-

1.      Rainbow trout (Salmo gairdneri)

2.      Channel catfish (Ictalurus punctatus) and

3.      Bluegill (Lepomis macrochirus)

 

The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.

 

The dye for the toxicity study was weighed separately for each vessel.

 

Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.

 

The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.

 

The toxicity study was performed at temperature of 21°C. LC50 value was determined at 24, 48 and 96 hrs.

 

Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-

 

Rainbow trout:LC50 values at

24 hrs - 4198 mg/l

48 hrs - 3420 mg/l

96 hrs –1372 mg/l

 

Channel catfish:LC50 values at

24 hrs -3828 mg/l

48 hrs -2826 mg/l

96 hrs –2267 mg/l

 

Bluegill:LC50 values at

24 hrs -5000 mg/l

48 hrs -4898 mg/l

96 hrs –3433 mg/l