Aluminium tridiethylphosphinate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

stirring (magnetic stirrer) for 24 h; adjusted to pH 7.0 to 7.2 and filtered;

Vehicle:

yes

Details on test solutions:

test water according to Elendt, M4; clear solution; no visible particulate matter after filtration;

Test organisms (species):

Daphnia magna

Details on test organisms:

feed three times a week with green algae; daphnids with an age < 24 h

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

mmol Ca2+ + Mg2+/l: 2.1 - 2.7

Test temperature:

21°C

pH:

7.0 - 7.2

Dissolved oxygen:

8.0 - 8.8 mg/l

Nominal and measured concentrations:

nominal 1mg/l; mean 1,014 mg/l
nominal 3,2mg/l; mean 3,250 mg/l
nominal 10mg/l; mean 10,049 mg/l
nominal 32mg/l; mean 31,907 mg/l
nominal 100mg/l; mean 98,410 mg/l

Details on test conditions:

100 ml beakers

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

ca. 46.2 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

dissolved

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

dissolved

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

ca. 32 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

dissolved

Basis for effect:

reproduction

Details on results:

All test animals in the 100 mg/ group died within 4 days. At 32 mg/l a mortality rate of 80% was observed. Daphnids of this group died between days 14 - 17 of the study. In the 1, 3.2 and 10 mg/l groups as well in the control group no mortality occurred.

Validity criteria fulfilled:

yes

Conclusions:

In this reproduction toxicity study with the test item in Daphnia magna over 21 days a NOECD (reproduction and mortality) of 10 mg/L were obtained. The EC10 reproduction is 12.8 mg/L, the EC10 mortality is 11.3 mg/L.

Executive summary:

The test item was tested in a daphnia magna reproduction study over 21 days in a semistatic system. The concentrations tested were 1, 3.2, 10, 32, 100 mg/L and 0 mg/L (control).

The test was conducted under semistatic conditions. Therefore, the test solutions were renewed every Monday, Wednesday and Friday using freshly prepared test solutions. The corresponding preparations were stirred for approx. 24 hours with a magnetic stirrer. To further improve solubility and stability of the test substance in the test medium, all test batches were adjusted to a pH range of 7.0 – 7.2 after the stirring period and run through a filter of 0.2 µm pore size.

All test solutions were present as clear solutions. There were no visible particulate matters. Water parameters were within the normal range.

Analytical monitoring of the concentrations of the test substance in the test medium was carried out during three test intervals. Sampling was performed at the start and the end of the interval. Two 48 hours- and one 72 hours interval were surveyed. The values determined were in a range of +/- 10% of the theoretical value.

In the control group, no mortality occurred and the mean number of living offspring produced per parent animal was 80.1, thus fulfilling the validity criteria for the test.

All test animals in the 100 mg/L group died within 4 days of exposure. At 32 mg/L a mortality rate of 80 % was observed. Daphnids of this group between days 14 – 17 of the study. In the 1, 3.2 and 10 mg/L groups, as well as in the control group, no mortality occurred.

Surviving animals of all concentration groups showed no difference in the onset of brood production in comparison to the control.

The reproduction rate in the 1-10 mg/L concentration groups showed no statistically significant changes in comparison to the control.

In this reproduction toxicity study with the test item in daphnia magna over 21 days the following effect concentrations were obtained:

 

Reproduction:

EC10 = 12.8 mg/L

EC50 = 46.2 mg/L

NOEC = 10 mg/L

 

Mortality

EC10 = 11.3 mg/L

EC50 = 22.3 mg/L

NOEC = 10 mg/L

Description of key information

In this reproduction toxicity study with the test item in Daphnia magna over 21 days a NOECD (reproduction and mortality) of 10 mg/L were obtained. The EC10 reproduction is 12.8 mg/L, the EC10 mortality is 11.3 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

10 mg/L