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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical 1-bromo-4-fluorobenzene on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance is sparingly soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) and then kept for 72 hours stirring. After the completion of 72 hours stirring, the stock solution was analytically detected and the solubility obtained from the analytical detection was then used for achieving test concentrations of 0.625 mg/L,1.25 mg/L,2.5 mg/L,5 mg/L & 10 mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.76 cm
- Weight at study initiation (mean and range, SD): 0.473g (average)
-Housing: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
152.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.03
Dissolved oxygen:
7.6 mg/l
Nominal and measured concentrations:
0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Other details not known
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: No effect were observed
Sublethal observations / clinical signs:

Table 1: Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

10

4

4

-

-

-

-

-

-

-

-

-

-

-

-

 

Table 3: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

4

4

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.625

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

1.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

2.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

10

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

               

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

Table 3. pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.68

7.69

8.07

8.08

8.22

8.23

8.29

8.28

0.625

4

4

7.71

7.74

8.10

8.11

8.20

8.21

8.21

8.22

1.25

4

4

7.76

7.77

8.20

8.21

8.23

8.22

8.25

8.26

2.5

4

4

7.79

7.81

8.22

8.23

8.24

8.25

8.29

8.28

5

4

4

7.85

7.87

8.25

8.26

8.26

8.28

8.30

8.31

10

4

4

7.89

7.90

8.27

8.28

8.29

8.29

8.33

8.34

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.1

7.1

6.3

6.3

6.0

6.0

6.0

6.0

0.625

4

4

7.0

7.0

6.1

6.1

5.7

5.7

5.3

5.3

1.25

4

4

7.0

7.0

6.1

6.1

5.7

5.7

5.3

5.3

2.5

4

4

7.0

7.0

6.1

6.1

5.9

5.9

5.2

5.2

5

4

4

7.0

7.0

6.1

6.1

5.8

5.8

5.4

5.4

10

4

4

7.0

7.0

6.3

6.3

5.9

5.9

5.5

5.5

Table: 4 Temperature

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.5

23.5

22.0

22.0

22.3

22.3

21.6

21.6

0.625

4

4

23.5

23.5

21.9

21.9

22.3

22.3

22.9

22.9

1.25

4

4

23.5

23.5

21.9

21.9

22.3

22.3

22.9

22.9

2.5

4

4

23.5

23.5

21.9

21.9

22.3

22.3

22.9

22.9

5

4

4

23.6

23.6

21.9

21.9

22.3

22.3

22.9

22.9

10

4

4

23.6

23.6

21.9

21.9

22.3

22.3

22.9

22.9
Validity criteria fulfilled:
yes
Conclusions:
After 96 hours of exposure to test item 1-bromo-4-fluoro benzene to various nominal test concentrations, LC50 was determine to be > 10 mg/l and No effect were observed at 10 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical 1-bromo-4-fluorobenzene on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance is sparingly soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) and then kept for 72 hours stirring. After the completion of 72 hours stirring, the stock solution was analytically detected and the solubility obtained from the analytical detection was then used for achieving test concentrations of 0.625 mg/L,1.25 mg/L,2.5 mg/L, 5 mg/L & 10 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 1-bromo-4-fluoro benzene to various nominal test concentrations, LC50 was determine to be > 10 mg/l and No effect were observed at 10 mg/l. Test consider to be valid as follows all parameter of OECD. Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. But as the chemical was readily biodegradable in water thus it does not exist for longer period of time. Thus on above criteria, chemical was consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical 1-bromo-4-fluorobenzene on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance is sparingly soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) and then kept for 72 hours stirring. After the completion of 72 hours stirring, the stock solution was analytically detected and the solubility obtained from the analytical detection was then used for achieving test concentrations of 0.625 mg/L,1.25 mg/L,2.5 mg/L, 5 mg/L & 10 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 1-bromo-4-fluoro benzene to various nominal test concentrations, LC50 was determine to be > 10 mg/l and No effect were observed at 10 mg/l. Test consider to be valid as follows all parameter of OECD. Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. But as the chemical was readily biodegradable in water thus it does not exist for longer period of time. Thus on above criteria, chemical was consider to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
10 mg/L

Additional information

Study was conducted to access the effect of test chemical 1-bromo-4-fluorobenzene on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance is sparingly soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) and then kept for 72 hours stirring. After the completion of 72 hours stirring, the stock solution was analytically detected and the solubility obtained from the analytical detection was then used for achieving test concentrations of 0.625 mg/L,1.25 mg/L,2.5 mg/L, 5 mg/L & 10 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 1-bromo-4-fluoro benzene to various nominal test concentrations, LC50 was determine to be > 10 mg/l and No effect were observed at 10 mg/l. Test consider to be valid as follows all parameter of OECD. Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria. But as the chemical was readily biodegradable in water thus it does not exist for longer period of time. Thus on above criteria, chemical was consider to be nontoxic and not classified as per the CLP classification criteria.