Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-046-0 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
OECD 201, GLP and Klimisch 1 algal inhibition 72 hour ErC50 2.63 mg/L, NOEC 0.5 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2.63 mg/L
- EC10 or NOEC for freshwater algae:
- 0.5 mg/L
Additional information
The toxicity of TOFA_DimerFA_TEPA_PAA (Batch no.: BB 000649 V1) to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD Guideline for Testing 201 (2006) at Dr.U.Noack-Laboratorien,31157 Sarstedt, Germany. The aim of the study was to assess the effects on the growth rate in a medium containing 50% natural river water and 50% algal culture medium over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 5089 cells/mL. Five concentration levels were tested in a geometrical series with a dilution factor of 2 (nominal): 0.50, 1.0, 2.0, 4.0 and 8.0 mg/L. Three replicates were tested for each concentration level and six replicates for the control. Environmental conditions (pH and room temperature) were determined to be within the acceptable limits.
TOFA_DimerFA_TEPA_PAA has low water solubility and may sorb to organic and inorganic materials by different mechanisms. Due to these properties the test item was difficult to test in artificial water (e.g. sorption to the test organism and walls of the test vessel). Natural river water contains particulate as well as dissolved organic carbon to which the test item can sorb partially preventing that the test item settles onto surfaces. The sorbed fraction of the test item was difficult to extract from the test system which normally leads to low analytical recoveries. Nevertheless the test item was present in the test system and therefore available for exposure (dissolved in water and sorbed).
No determinations of the test item concentrations were carried out. Preliminary investigations including the use of different analytical techniques have shown that no specific method was available that will meet the required sensitivity in the range of the test concentrations.
All effect levels are given based on nominal concentrations of TOFA_DimerFA_TEPA_PAA.
The 72 h ErC50 of TOFA_DimerFA_TEPA_PAA he freshwater green alga Pseudokirchneriella subcapitata was 2.63 mg/L (95% confidence limits of 2.49 – 2.80 mg/L) and the corresponding NOEC was 0.5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
