[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016/01/14 - 2016/02/25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to a suitable and standardized test method under GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

EC Number:

244-096-4

EC Name:

[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

Cas Number:

20882-04-6

Molecular formula:

C10H14O6

IUPAC Name:

4-{2-[(2-methylprop-2-enoyl)oxy]ethoxy}-4-oxobutanoic acid

Constituent 2

Reference substance name:

[SPEC][/SPEC][SYN][/SYN]

IUPAC Name:

[SPEC][/SPEC][SYN][/SYN]

Constituent 3

Reference substance name:

[CS]000000111817[/CS]

IUPAC Name:

[CS]000000111817[/CS]

Test material form:

other: liquid

Details on test material:

Company internal substance number SAT 150073, Batch4006/31
Colorless liquid at room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
The samples were diluted by a factor of two with acetonitrile.
Additional samples of the control and the dilution solvent were taken at test start and test end without any sample treatment.
The concentrations of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate were analysed in the duplicate test media samples from all test concentrations, and in the duplicate control samples, from both sampling times (0 and 48 hours).

Test solutions

Vehicle:

no

Details on test solutions:

Test Concentrations: 515.4, 234.3, 106.5, 48.4 and 22.0 mg test item/L and a control

Control:
In the control, test water was used without addition of the test item.

Dosage of Test Item:
The test medium of the highest test concentration of nominal 515.4 mg test item/L was prepared by dissolving 462.8 mg test item into 898 mL test water by intense stirring for 10 minutes. Adequate volumes of this test medium were diluted with test water to prepare the test media.
The test media were prepared just before introduction of the Daphnia (= start of the test).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna (Straus), clone 5

Age at Test Start: From 2 to 17 hours old

Sex: Female

Origin: The Daphnia introduced in the test were taken from ibacon's in-house laboratory culture.

Breeding Conditions: The Daphnia were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental Daphnia was performed in Elendt M4 medium (see 6.5). The test organisms were not first brood progeny. The Daphnia in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

20 °C at test start; 20 °C at test end

pH:

pH-Values:
8.0 to 8.5 at test start; 7.7 to 8.2 at test end

Dissolved oxygen:

8.8 to 9.3 mg/L at test start; 7.7 to 8.0 mg/L at test end

Nominal and measured concentrations:

Summary of Analytical Results
Sample Description % of RSD
[mg test item/L] nominal1 [%] n
Control (Control blank) n.a. n.a. 2
Dilution Solvent n.a. n.a. 2
Control n.a. n.a. 4
22.0 107 1 4
48.4 111 3 4
106.5 106 2 4
234.3 105 1 4
515.4 108 1 4
1 mean value of all measured samples per treatment group
RSD: relative standard deviation per treatment group
n: number of analysed samples
n.a.: not applicable

Details on test conditions:

Test Units
Type and Size:
Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.

Identification:
Each test unit was uniquely identified with serial number, treatment and replicate number.

Test Environment:
Controlled environment room

Measurement of pH, Dissolved Oxygen and Water Temperature:
The water temperature, pH-values and dissolved oxygen concentrations were determined at test start and test end in each treatment group.

Reference substance (positive control):

yes

Remarks:

For the evaluation of the quality of the Daphnia clone and the consistency of the experimental conditions, the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 515.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 515.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 515.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be ≥515.4 mg test item/L. The 48-hour LOEC was determined to be > 515.4 mg test item/L and the 48-hour EC50 value was determined to be >515.4 mg test item/L.

Executive summary:

The toxic effect of the test item [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be ≥515.4 mg test item/L. The 48-hour LOEC was determined to be > 515.4 mg test item/L and the 48-hour EC50 value was determined to be >515.4 mg test item/L.