Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization Study of test material on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2

pH:

7.4

Nominal and measured concentrations:

6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: No immobility was observed

9.1. Immobility

Nominal	Number	of	Immobility
Concentration	Daphnids	0 h	24 h		48 h
(mg/l)
Control	10	-	-		-
6.25	10	-	-		-
12.5	10	-	-		-
25	10	-	-		-
50	10	-	-		-
100	10	-	-		-

9.2. pH and oxygen content:

Nominal Concentration Number of			pH(mg/L)
(mg/l)		daphnids	0h	48h
Control		10	7.2	7.3
6.25		10	7.2	7.3
12.5		10	7.3	7.4
25		10	7.4	7.5
50		10	7.4	7.5
100		10	7.5	7.6

Nominal Concentration Number of			DO(mg/L)
(mg/l)		daphnids	0h	48h
Control		10	7.1	6.9
6.25		10	7.1	6.9
12.5		10	7.1	6.9
25		10	7.1	6.9
50		10	7.1	6.9
100		10	7.1	6.9

9.3. Test temperature:

Nominal Concentration Number of				Temparature
	daphnids	0h	48h
	10	20.1	20.1
	10	20.1	20.1
	10	20.1	20.1
	10	20.1	20.1
	10	20.1	20.1
	10	20.1	20.1

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be > 100 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.

The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.

The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

The short term toxicity to aquatic invertebrate for the test material was determined based on the data derived from two experimental reports .

In one report , Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media having concentration 1g/L. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L, respectively.

The nominal concentration selected for the experiment were 6.25 mg/L,12.5 mg/L,25 mg/L, 50 mg/L and 100 mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the test substance, does not exhibit short term toxicity to Daphnia.After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

In another report Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 100.0 mg/l was prepared by dissolving dark red powder in reconstituted water. The test solutions of required comcentrations were prepared by mixing the stock solution of the test sample in reconstituted water.12 , 20 , 32 , 50 , 80 , 100 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 51.9 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

The effect concentration determined by both the study reports are contradictory to each other since , value of the first report is from recently performed study we would consider that the test material is not classified as per CLP criteria.