Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: Chemicals-Adsorption-Desorption Using a Batch Equilibrium Method (GB/T21851-2008)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.18 (Adsorption / Desorption Using a Batch Equilibrium Method)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: The Guidelines for the Testing of Chemicals, Physico-Chemical Properties and Physical Hazards (the 2nd edition). Beijing: China Environmental Press
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)
Deviations:
no
GLP compliance:
yes
Type of method:
batch equilibrium method
Media:
soil

Test material

Constituent 1
Test material form:
liquid
Details on test material:
- Purity: >99%
Radiolabelling:
no

Study design

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: UPLC ACQUITY
- Column: 1.8 µm 2.1 x 100 mm Column
- Detection system: ACQUITY UPLC PDA Detector
- Wavelength : 210 nm
- Column temperature: 30°C
- Mobile phase: Acetonitrile: 0.1 % formic acid: 55:45
- Flow rate: 0.3 mL/min
- Inject volume: 10 µL
- Retention time: 1.9 min

Batch equilibrium or other method

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0.50, 1.00, 2.00, 5.00, 10.0 and 20.0 mg/L
- Sample storage before analysis: Room temperature

Results and discussion

Adsorption coefficientopen allclose all
Key result
Sample No.:
#1
Type:
Kd
Value:
27.3 other: cm3/g
Key result
Sample No.:
#2
Type:
Kd
Value:
21.6 other: cm3/g
Key result
Sample No.:
#3
Type:
Kd
Value:
96 other: cm3/g
Key result
Sample No.:
#4
Type:
Kd
Value:
20.1 other: cm3/g
Key result
Sample No.:
#5
Type:
Kd
Value:
53.4 other: cm3/g
Key result
Sample No.:
#1
Type:
Koc
Value:
417 other: cm3/g
Key result
Sample No.:
#2
Type:
Koc
Value:
1 597 other: cm3/g
Key result
Sample No.:
#3
Type:
Koc
Value:
1 792 other: cm3/g
Key result
Sample No.:
#4
Type:
Koc
Value:
1 448 other: cm3/g
Key result
Sample No.:
#5
Type:
Koc
Value:
780.2 other: cm3/g

Results: Batch equilibrium or other method

Adsorption and desorption constants:
The results of adsorption kinetic experiments show that the adsorption coefficient (Kd) values of test substance in A, B, C, D, E soil were 27.3, 21.6, 96.0, 20.1 and 53.4 cm3/g, respectively. And the Koc values of test substance were 417.0, 1597, 1792, 1448 and 780.2 cm3/g, respectively.
The results of desorption kinetic experiments show that during the appropriate desorption equilibrium time of 48 h, the test substance was not detected in the supernatant of A B, C, D, E soil
The results of adsorption isotherms experiments show the Freundlich adsorption coefficient values in five soils were 154, 148, 227, 113 and 213, respectively. The 1/n value for different soils was 0.269-0.365.
The results of desorption isotherms experiments show that during the appropriate desorption equilibrium time of 48 h, the test substance was not detected in the supernatant of A, B, C, D, E soil.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Adsorption coefficient (Kd): 27.3, 21.6, 96.0, 20.1 and 53.4 cm3/g; Koc: 417.0, 1597, 1792, 1448 and 780.2 cm3/g.
Test substance was not detected in the supernatant of different type of soil for desorption.
Executive summary:

According to "The guidelines for the testing of chemicals" (HJ/T 153-2004) and "The Guidelines for the Testing of Chemicals-Physical-Chemical Properties and Physical Hazards" (the 2nd edition. Beijing: China Environment Press. 2013), with reference to Procedure 106 of the "Guidelines for Testing of Chemicals" of the OECD: "Adsorption-Desorption Using a Batch Equilibrium Method" (2000), the adsorption-desorption test for test substance was conducted for generating essential information of biogeocoenosis for new chemicals registration. Five soil types with different pH, texture, and organic carbon contents sampling from Jilin, Jiangxi, Jiangsu, Shandong and Gansu provinces of China were selected. Batch equilibrium method was adopted, and tests were performed to determine the adsorption characteristics of test substance.

The results show that optimal soil/aqueous ratio for the five soils were 1:100, and the appropriate adsorption equilibrium time was 24 h.

The results of adsorption kinetic experiments show that the adsorption coefficient (Kd) values of test substance in A, B, C, D, E soil were 27.3, 21.6, 96.0, 20.1 and 53.4 cm3/g, respectively. And the Koc values of test substance were 417.0, 1597, 1792, 1448 and 780.2 cm3/g in above soils respectively.

The results of desorption kinetic experiments show that during the appropriate desorption equilibrium time of 48 h, the test substance was not detected in the supernatant of A B, C, D, E soil.

The results of adsorption isotherms experiments show the Freundlich adsorption coefficient values in five soils were 154, 148, 227, 113 and 213, respectively. The 1/n value for different soils was 0.269-0.365.

The results of desorption isotherms experiments show that during the appropriate desorption equilibrium time of 48 h, the test substance was not detected in the supernatant of A, B, C, D, E soil.