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EC number: 618-226-9 | CAS number: 889652-22-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (4aR,4bS,6aS,7S,7aS,8aS,8bR,8cR)-7-[2-(1,3-dioxolan-2-yl)ethyl]-2-methoxy-4a,6a-dimethyl-3,4,4a,4b,5,6,6a,7,7a,8,8a,8b,8c,9-tetradecahydrocyclopropa[4,5]cyclopenta[1,2-a]phenanthren-7-ol
- EC Number:
- 618-226-9
- Cas Number:
- 889652-22-6
- Molecular formula:
- C26H38O4
- IUPAC Name:
- (4aR,4bS,6aS,7S,7aS,8aS,8bR,8cR)-7-[2-(1,3-dioxolan-2-yl)ethyl]-2-methoxy-4a,6a-dimethyl-3,4,4a,4b,5,6,6a,7,7a,8,8a,8b,8c,9-tetradecahydrocyclopropa[4,5]cyclopenta[1,2-a]phenanthren-7-ol
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- The test organism was grown and hold in OECD medium which was used also for preparing test item solutions.
The Cyclopropyl-enolether-acetal has a very low solubility in aquatic solutions so its concentration cannot be determined by UHPLC from aquatic solutions directly.
The stock solution was prepared by measuring 200.18 mg of test item into 2 000 ml OECD growth medium. After 15 minutes ultrasonication and two hours stirring the suspension was filtered through 0.2 μm WhatmannTM ME24 filter. The concentration of the filtrate (stock solution) was 0.136 mg/L.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum and Raphidocelis subcapitata)
Source: Culture Collection of Algae and Protozoa, Scottish Marine
Institute (www.ccap.ac.uk)
Batch: CCAP 278/4
Date of arriving: 27 October 2017
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Nominal and measured concentrations:
- The stock solution was prepared by measuring 200.18 mg of test item into 2 000 ml OECD growth medium. After 15 minutes ultrasonication and two hours stirring the suspension was filtered through 0.2 μm WhatmannTM ME24 filter. The concentration of the filtrate (stock solution) was 0.136 mg/L.
The toxic property of test item was investigated at five concentrations which were prepared by different dilutions of stock solution of test item. OECD medium was used for dilution of stock solution. In the test the following measured and calculated concentrations were applied: 0.136; 0.091; 0.068; 0.045 and 0.034 mg/L. - Details on test conditions:
- At each concentration level three replicates were used. In the case of control six replicates were applied. The initial alga concentration was 4 973 cells/ml. The vessels were closed with silicone stoppers with a filter for ensuring the proper air exchange. The vessels were kept in incubator at 21 ± 2 °C with continuous illumination. The treatments of control and test vessels were identical.
The test fulfilled all of the validity criteria required by the OECD 201 guideline.
The statistical evaluation in the test based on calculated concentrations. The number of alga cells was determined daily by microscopically counting with Bürker chamber.
The ECx for specific growth rate and yield were determined by ToxRat® statistic software.
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks on result:
- not determinable
- Details on results:
- The ECx for specific growth rate and yield were determined by ToxRat® statistic software. The following values were calculated after 72 hours:
ECx values For yield an for specific growth rate
EC10: Not determined
EC20: Not determined
EC50: Not determined
According to the test results Cyclopropyl-enolether-acetal has no toxic effect on alga growth rate even at its saturated concentration (0.136 mg/L).
Applicant's summary and conclusion
- Conclusions:
- According to the test results Cyclopropyl-enolether-acetal has no toxic effect on alga growth rate even at its saturated concentration (0.136 mg/L).
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