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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(4aR,4bS,6aS,7S,7aS,8aS,8bR,8cR)-7-[2-(1,3-dioxolan-2-yl)ethyl]-2-methoxy-4a,6a-dimethyl-3,4,4a,4b,5,6,6a,7,7a,8,8a,8b,8c,9-tetradecahydrocyclopropa[4,5]cyclopenta[1,2-a]phenanthren-7-ol
EC Number:
618-226-9
Cas Number:
889652-22-6
Molecular formula:
C26H38O4
IUPAC Name:
(4aR,4bS,6aS,7S,7aS,8aS,8bR,8cR)-7-[2-(1,3-dioxolan-2-yl)ethyl]-2-methoxy-4a,6a-dimethyl-3,4,4a,4b,5,6,6a,7,7a,8,8a,8b,8c,9-tetradecahydrocyclopropa[4,5]cyclopenta[1,2-a]phenanthren-7-ol
Test material form:
solid: crystalline

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
The test organism was grown and hold in OECD medium which was used also for preparing test item solutions.
The Cyclopropyl-enolether-acetal has a very low solubility in aquatic solutions so its concentration cannot be determined by UHPLC from aquatic solutions directly.
The stock solution was prepared by measuring 200.18 mg of test item into 2 000 ml OECD growth medium. After 15 minutes ultrasonication and two hours stirring the suspension was filtered through 0.2 μm WhatmannTM ME24 filter. The concentration of the filtrate (stock solution) was 0.136 mg/L.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum and Raphidocelis subcapitata)
Source: Culture Collection of Algae and Protozoa, Scottish Marine
Institute (www.ccap.ac.uk)
Batch: CCAP 278/4
Date of arriving: 27 October 2017

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
The stock solution was prepared by measuring 200.18 mg of test item into 2 000 ml OECD growth medium. After 15 minutes ultrasonication and two hours stirring the suspension was filtered through 0.2 μm WhatmannTM ME24 filter. The concentration of the filtrate (stock solution) was 0.136 mg/L.
The toxic property of test item was investigated at five concentrations which were prepared by different dilutions of stock solution of test item. OECD medium was used for dilution of stock solution. In the test the following measured and calculated concentrations were applied: 0.136; 0.091; 0.068; 0.045 and 0.034 mg/L.
Details on test conditions:
At each concentration level three replicates were used. In the case of control six replicates were applied. The initial alga concentration was 4 973 cells/ml. The vessels were closed with silicone stoppers with a filter for ensuring the proper air exchange. The vessels were kept in incubator at 21 ± 2 °C with continuous illumination. The treatments of control and test vessels were identical.
The test fulfilled all of the validity criteria required by the OECD 201 guideline.
The statistical evaluation in the test based on calculated concentrations. The number of alga cells was determined daily by microscopically counting with Bürker chamber.
The ECx for specific growth rate and yield were determined by ToxRat® statistic software.

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Remarks on result:
not determinable
Details on results:
The ECx for specific growth rate and yield were determined by ToxRat® statistic software. The following values were calculated after 72 hours:
ECx values For yield an for specific growth rate
EC10: Not determined
EC20: Not determined
EC50: Not determined
According to the test results Cyclopropyl-enolether-acetal has no toxic effect on alga growth rate even at its saturated concentration (0.136 mg/L).

Applicant's summary and conclusion

Conclusions:
According to the test results Cyclopropyl-enolether-acetal has no toxic effect on alga growth rate even at its saturated concentration (0.136 mg/L).