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EC number: 201-553-2 | CAS number: 84-69-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted as part of the US National Toxicology Program. Article published in established peer-reviewed journal; standard protocol followed.
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Phthalate ester testing in the National Toxicology Program’s Environmental Mutagenesis Test Development Program
- Author:
- Zeiger, E., Haworth, S., Speck, W., and Mortelmans, K.
- Year:
- 1 982
- Bibliographic source:
- Environmental Health Perspectives, 45, 99-101, 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diisobutyl phthalate
- EC Number:
- 201-553-2
- EC Name:
- Diisobutyl phthalate
- Cas Number:
- 84-69-5
- Molecular formula:
- C16H22O4
- IUPAC Name:
- diisobutyl phthalate
- Details on test material:
- Reagent grade
Constituent 1
Method
- Target gene:
- Histidine prototrophy assay in Salmonella typhimurium (Ames test)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9/cofactors (exogenous metabolic activation
- Test concentrations with justification for top dose:
- 100-10,000 µg/plate (recommended maximum for OECD 471 is 5,000 µg/plate)
- Vehicle / solvent:
- DIBP dissolved in DMSO.
- Details on test system and experimental conditions:
- Slight deficiency in number/type of bacterial strains with respect to OECD 471.
Doses/concentration: 100-10,000 µg/plate (recommended maximum for OECD 471 is 5,000 µg/plate).
Pre-incubation modification of the method of Ames et al.
Bacteria were tested for reversion to histidine prototrophy, both in the absence and presence of S9/cofactors (exogenous metabolic activation system). Concurrent positive and solvents controls were included to demonstrate the effective performance of the assays. - Evaluation criteria:
- Testing conducted in a blind manner. Decoding of samples performed by NTP after data evaluation.
Concurrent positive and solvents controls were included to demonstrate the effective performance of the assays.
Treatment of data: A positive mutagenic response was defined as a reproducible, dose-related increase in his+ revertants over the spontaneous level.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type:
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
DIBP was not mutagenic in this assay, neither in the absence nor presence of an exogenous metabolic activation system. - Executive summary:
Bacteria were tested for reversion to histidine prototrophy, both in the absence and presence of S9/cofactors (exogenous metabolic activation system). Doses/concentration:100-10,000 µg/plate. Pre-incubation modification of the method of Ames et al. Concurrent positive and solvents controls were included to demonstrate the effective performance of the assays. DIBP was not mutagenic in this assay, with and without metabolic activation.
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