Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: conducted to OECD/EC guidelines with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): TK13061/2
- Physical state: Solid
- Lot/batch No.: LIC 613/04
- Storage condition of test material: room temperature in the dark

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Green algae
- Strain: CCAP 276/20
- Source (laboratory, culture collection): CCAP, Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
24 +/- 1 C
Nominal and measured concentrations:
Range finding test: nominal concentrations of 0.1, 1.0, 10 and 100 mg/L were exposed to Desmodesmus subspicatus for a period of 72 hours.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 ml
- Aeration: none
- Initial cells density: 4E3 (nominal)
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

OTHER TEST CONDITIONS
- Photoperiod: 72 hours
- Light intensity and quality: 7000 lux provided by warm white lighting (380 - 730 nm)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter Multisizer particle counter


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 100 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Remarks:
cell number and biomass
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Remarks:
cell number and biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Remarks:
cell number and biomass
Details on results:
Range finding test concluded that a limit test had to be carried out.
Results with reference substance (positive control):
- Results with reference substance valid? yes
- ErC50: 0.64 mg/L
- EyC50: 0.36 mg/L
- EbC50: 0.32 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The effect of the test material on the growth of Desmodesmus subspicatus has been investigated and gave EC50 values of greater than 100 mg/L. Correspondingly the NOEC was 100 mg/L.
Executive summary:

In a 72 hour acute toxicity study, the cultures of Desmodesmus subspicatus were exposed to Trisodium [carboxy(oxido)methyl]phosphonate at nominal concentrations of 100 mg a.i./L under static conditions in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test).  The EC50 value was >100 Mg a.i./L. 

 

Results Synopsis

 

Test Organism: Desmodesmus subspicatus

Test Type (Flowthrough, Static, Static Renewal): static

 

72 hr EC50: > 100 mg a.i./L