hexasodium (R)-carboxy(phosphono)methanolate (S)-carboxy(phosphono)methanolate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test material is 50% aqueous of submission substance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC guideline L251

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Belcor 575 (TK13061)
- Physical state: Brown liquid
- Analytical purity: 52.5%
- Lot/batch No.: P807040
- Expiration date of the lot/batch: 06/1993
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: Daphnia magna
- Source: CIBA-GEIGY Ltd., Facilities, Basle
- Age at study initiation (mean and range, SD): 6 - <24h
- Method of breeding: Cultures of daphnia are maintained in glass vessels containing approximately 2.5 L of reconstitued water at 20 +/- 1 C (water is renewed partiallt thrice weekly). At each renewal the daphnia are fed a suspension of green algae (scenedesmus subspicatus) supplemented by a suspension of tetramin-extract in such quantities that the food is consumed after 24 hours.
- Feeding during test: none during the test

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Test conditions

Hardness:

240 mg CaCO2/L

Test temperature:

20 +/- 1 C

pH:

5.3 - 7.9

Dissolved oxygen:

102 - 105 %

Nominal and measured concentrations:

10, 18, 32, 58, 100, 180, 320, 580, 1000 mg/L (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 100 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


OTHER TEST CONDITIONS
- Photoperiod:Fluorescent light, 16 hous daily
- Light intensity: 1500 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

230 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

580 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Details on results:

Immobilization in control - 0%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

24 hour EC50 = 230 mg/L (nominal)
EC0 = 100 mg/L (nominal)
EC100 = 580 mg/L (nominal)

Executive summary:

The 24h acute toxicity of Trisodium [carboxy(oxido)methyl]phosphonate to Daphnia magna was studied under static conditions.  Daphnids were exposed to control, solvent control, and test chemical at nominal concentrations of 10, 18, 32, 58, 100, 180, 320, 580, 1000 mg/L for 24 h.  Mortality/immobilization and sublethal effects were observed.  The 24 hour EC₅₀ was 230 mg a.i./L. 

 

Results Synopsis

 

Test Organism Age: <24 h

Test Type (Flowthrough, Static, Static Renewal): Static

 

EC₅₀:  230 mg a.i./L