Tetrasodium {5-[(4-chloro-6-{[4-(alkenylsulfonyl)phenyl]amino}-heteromonocycl-2-yl)amino]-4-(hydroxy-κO)-3-{[2-(hydroxy-κO)phenyl]diazenyl-κN}naphthalene-disulfonato(4-)}[3-(hydroxy-κO)-4-{[2-(hydroxy-κO)naphthalen-1-yl]diazenyl-κN}naphthalene-sulfonato(3-)]chromate(4-)

Short-term toxicity to aquatic invertebrates

Description of key information

Determination of the acute toxicity of the test item, FAT 20341/A TE, to Daphnia magna

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

53 mg/L

Additional information

The study was performed to assess the acute toxicity of the test item to Daphnia magna.

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at concentrations of 1.0, 3.2, 10, 32 and 100 mg/L for 48 hours at a temperature of approximately 20°C under static test conditions. The number of immobilized daphnia were recorded after 24 and 48 hours.

 

Results:

Analysis of the 10, 32 and 100 mg/L test preparations at 0 and 48 hours showed measured test concentrations to range from 94% to 99% of nominal value and so the results are based on nominal test concentrations only.

 

Exposure ofDaphnia magnato the test item gave the following results:

 

Time Point (Hours)	EC50 (mg/L)	95% Confidence Limits (mg/L)			No Observed Effect Concentration (NOEC) (mg/L)	Lowest Observed Effect Concentration (LOEC) (mg/L)
48	53	48	-	60	10	32