Disodium 2-[[5-carbamoyl-1-ethyl-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridyl]azo]-4-[[4-[(2-chloro-5-sulphonatophenyl)amino]-6-fluoro-1,3,5-triazin-2-yl]amino]benzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 January 1993 to 13 March 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 4 April 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

February 1990

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT 40138/D
Batch No.: 275605.26
Purity: 79 %
Appearance: solid
Color: yellow
Solubility: 100 g/l [in water]
Storage: at room temperature
Expiration date: July, 1993

Analytical monitoring:

yes

Details on sampling:

For the analytical measurements of the test substance concentrations duplicate samples from the freshly prepared test solution and the control were taken at the start of the test. For the determination of the stability of the test substance under test conditions, samples from the test media were collected at the end of the test (after 48 hours). The concentration of the test substance FAT 40138/D was analysed in singular in the control samples and in the duplicate test media samples from the nominal test concentration of 1000 mg/l at both sampling dates (0 and 48 hours). All samples are kept stored at -20 °C to enable additional analyses.

Vehicle:

no

Details on test solutions:

The test substance was weighed and directly dissolved in the test water just before introduction of daphnids ( = start of the test). The only nominal concentration tested was 1000 mg test substance/l and a control (test water without any additions).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: RCC (testing laboratory)

The study has been performed with young daphnids (at the start of the test 6 - 24 hours old) of a clone of the species Daphnia magna Straus. The clone was bred in the laboratories of RCC under standardized conditions. The cultivation of the parental daphnids was performed in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.

Test type:

static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/l CaCO3

Test temperature:

19.4 - 20.0 °C

pH:

7.7 - 7.9

Dissolved oxygen:

9.2 mg/l or higher

Nominal and measured concentrations:

1000 mg test article/l (nominal concentration);

Details on test conditions:

Experimental conditions:
In the test concentration and control 20 daphnids were tested, divided into four groups of five animals, each group in 50 ml test medium in a glass-beaker (100 ml). The daphnids were not fed during the test period. The test was performed in an air-conditioned room.

Water temperature: 19.4 - 20.0 °C during test period.

Light: 16 hours per day, approximately 200 - 600 Lux

Test duration: 48 hours

Test water: reconstituted water: in deionized water with a conductivity lower than 0.1 µS cm-1 analytical grade salts were dissolved and the water was aerated to saturation.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The measured test substance concentrations in the test solution amounted to 103.9 %, respectively, 104.9 % of the nominal value. The test substance was sufficiently stable under test conditions during the test period. Therefore, the reported results are related to the nominal concentrations of the test substance. No immobility or mortality was observed in the control and in the test solution of 1000 mg test solution/l during the test.

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 of FAT 40138/D in the acute toxicity to daphnia was calculated as >1000 mg/l.

Executive summary:

The acute toxicity of FAT 40138/D to Daphnia magna was determined in a 48 hours static test according to the OECD Guideline 202 and EU Method C.2. Based on the results of a pretest the nominal concentration for the main test was selected as 1000 mg test article/l.

No immobility or mortality was observed in the control and in the test solution of 1000 mg test solution/l during the test. Thus, the NOEC and EC0 value after 48 hours were determined to be 1000 mg/l. The EC50 value after 48 hours was determined as >1000 mg/l.

Description of key information

The EC50 value after 48 hours was determined as >1000 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L

Additional information

In a key study, the acute toxicity of FAT 40138/D to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline 202 and EU Method C.2. Based on the results of a pretest the nominal concentration for the main test was selected as 1000 mg test article/l. No immobility or mortality was observed in the control and in the test solution of 1000 mg test solution/l during the test. Thus, the 48 h NOEC and EC0 value were determined to be 1000 mg/l. The EC50 value after 48 hours was determined as >1000 mg/l.