Disodium 2-[[5-carbamoyl-1-ethyl-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridyl]azo]-4-[[4-[(2-chloro-5-sulphonatophenyl)amino]-6-fluoro-1,3,5-triazin-2-yl]amino]benzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

16 October 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

other: Internal method " Method A-11, Acute Fish Toxicity"

Version / remarks:

Equivalent to OECD Guideline 203

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Details on sampling:

None

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

other: Rainbow trout also known as Oncorhynchus mykiss (previous name: Salmo gairdneri); Zebra fish also known as Danio rario

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

None

Test temperature:

Rainbow trout: 16 °C
Zebra fish: 25 °C

pH:

Rainbow trout: 7.8 - 8.1
Zebra fish: 8 - 8.1

Dissolved oxygen:

Rainbow trout: 8.3 - 8.8 mg/L
Zebra fish: 6.5 mg/L (only one reading taken. this reading was for the concentration 300 mg/L)

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

Rainbow trout: 100, 300 and 1000 mg/L
Zebra fish: 100, 300 and 1000 mg/L

Details on test conditions:

- No of fish tested for each concentration: 8 each for rainbow trout and 10 each for zebra fish
Refer " Any other information on material and methods incl. tables" for details

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

260 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: For test with rainbow trout

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

334 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: For test with Zebra fish

Details on results:

Test with rainbow trout: No mortality was observed at the concentration of 100 mg/L. Three out of 8 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. The 48 h and 96 h LD0 and LD100 of FAT 40138/A was 100 and 1000 mg/L, respectively.

Test with zebra fish: No mortality was observed at the concentration of 100 mg/L. One out of 10 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. The 48h and 96 h LD0 and LD 100 of FAT 40138/A was 100 and 1000 mg/L, respectively.

Validity criteria fulfilled:

not specified

Conclusions:

LC50 for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.

Executive summary:

The acute fish toxicity of FAT 65042/C was assessed in a study conducted acccording to the internal Ciba method " Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. In the test with rainbow trout, three groups of 8 fish each were exposed to 100, 300 and 1000 mg/l respectively for 96 hours. In the test with Zebra fish, three groups of 10 zebra fish each, were exposed to test concentrations of 100, 300 and 1000 mg/L for 96 hours. In the test with rainbow trout, no mortality was observed at the concentration of 100 mg/L, three out of 8 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. Similarly, in the test with zebra fish, no mortality was observed at the concentration of 100 mg/L, one out of 10 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. Hence, the LC₅₀ for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.

Description of key information

The 96 h LC₅₀ of test substance in rainbow trout fish was determined to be 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

Toxicity potential of FAT 40138 based on short-term exposure was assessed with the help of two studies. In a key study, acute fish toxicity of FAT 40138/A was assessed according to the internal Ciba method " Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. In the test with rainbow trout, three groups of 8 fish each were exposed to 100, 300 and 1000 mg/L for 96 h, respectively. In the test with zebra fish, two groups of 8 zebra fishes each, were exposed to test concentrations of 300 and 1000 mg/L for 96 h. No mortality was observed throughout the observation period with rainbow trout as well as zebra fish. Hence, the LC₅₀ for test substance was determined to be >1000 mg/L. In a supporting study, the 96 h LC₅₀ of FAT 40138/C for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.