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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
16 October 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
other: Internal method " Method A-11, Acute Fish Toxicity"
Version / remarks:
Equivalent to OECD Guideline 203
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
no
Details on sampling:
None
Vehicle:
not specified
Details on test solutions:
None
Test organisms (species):
other: Rainbow trout also known as Oncorhynchus mykiss (previous name: Salmo gairdneri); Zebra fish also known as Danio rario
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
None
Test temperature:
Rainbow trout: 16 °C
Zebra fish: 25 °C
pH:
Rainbow trout: 7.8 - 8.1
Zebra fish: 8 - 8.1
Dissolved oxygen:
Rainbow trout: 8.3 - 8.8 mg/L
Zebra fish: 6.5 mg/L (only one reading taken. this reading was for the concentration 300 mg/L)
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
Rainbow trout: 100, 300 and 1000 mg/L
Zebra fish: 100, 300 and 1000 mg/L
Details on test conditions:
- No of fish tested for each concentration: 8 each for rainbow trout and 10 each for zebra fish
Refer " Any other information on material and methods incl. tables" for details
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
260 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: For test with rainbow trout
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
334 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: For test with Zebra fish
Details on results:
Test with rainbow trout: No mortality was observed at the concentration of 100 mg/L. Three out of 8 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. The 48 h and 96 h LD0 and LD100 of FAT 40138/A was 100 and 1000 mg/L, respectively.

Test with zebra fish: No mortality was observed at the concentration of 100 mg/L. One out of 10 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. The 48h and 96 h LD0 and LD 100 of FAT 40138/A was 100 and 1000 mg/L, respectively.
Validity criteria fulfilled:
not specified
Conclusions:
LC50 for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.
Executive summary:

The acute fish toxicity of FAT 65042/C was assessed in a study conducted acccording to the internal Ciba method " Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. In the test with rainbow trout, three groups of 8 fish each were exposed to 100, 300 and 1000 mg/l respectively for 96 hours. In the test with Zebra fish, three groups of 10 zebra fish each, were exposed to test concentrations of 100, 300 and 1000 mg/L for 96 hours. In the test with rainbow trout, no mortality was observed at the concentration of 100 mg/L, three out of 8 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. Similarly, in the test with zebra fish, no mortality was observed at the concentration of 100 mg/L, one out of 10 fish were found dead at the concentration of 300 mg/L, while 100 % mortality was observed with 1000 mg/L concentration. Hence, the LC50 for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.

Description of key information

The 96 h LC50 of test substance in rainbow trout fish was determined to be 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Additional information

Toxicity potential of FAT 40138 based on short-term exposure was assessed with the help of two studies. In a key study, acute fish toxicity of FAT 40138/A was assessed according to the internal Ciba method " Method A-11, Acute Fish toxicity", which is equivalent to OECD Guideline 203. In the test with rainbow trout, three groups of 8 fish each were exposed to 100, 300 and 1000 mg/L for 96 h, respectively. In the test with zebra fish, two groups of 8 zebra fishes each, were exposed to test concentrations of 300 and 1000 mg/L for 96 h. No mortality was observed throughout the observation period with rainbow trout as well as zebra fish. Hence, the LC50 for test substance was determined to be >1000 mg/L. In a supporting study, the 96 h LC50 of FAT 40138/C for rainbow trout and zebra fish was determined to be 260 and 334 mg/L, respectively.