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EC number: 813-338-6 | CAS number: 106155-02-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- study conducted under GLP and according to DIN 38412 part 27 (1992). According to ECHA endpoint specific guidance chapter 7b (2012), subchapter toxicty tests with bacteria (page 149), "In general, preference is given to tests with a mixed inoculum that assess the functioning of the entire microbial community in an STP, rather than tests based on single species or even microbial sub-systems ... Often inhibition test data on individual bacterial species may be available. Results of the cell multiplication inhibition test with P. putida (Bringmann and Kühn 1980) should be used for calculation of the PNECmicro-organisms only in cases where no other test results are available."
- Qualifier:
- according to guideline
- Guideline:
- other: DIN-38412, part 27 (1992)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations:
Preparation of test water by direct weighing of the test substance into the test vessels and ultrasonic treatment before the bacteria suspension was added
- Sampling method: not reported
- Sample storage conditions before analysis: not applicable - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing and mixing into test vessel and ultrasonic treatment
- Eluate: not applicable
- Differential loading: not applicable
- Controls: yes (blind values)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): up to and including 10 mg/L clear solution, turbid suspension at higher test concentration with an observable separation of phases - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: TBB-Microbiology, Henkel KGaA
- Method of cultivation: not reported
- Preparation of inoculum for exposure: 1-3 mL bacterial suspension added to the test solution composed of test water (according to DIN 38412, part 27. point 8.1), 2 mL Glucose solution and test substance
- Pretreatment: bacterial suspension 2-3 days old
- Initial biomass concentration: not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3.8 min
- Remarks on exposure duration:
- observation times different in the treatments dependent on the decrease rate of oxygen
- Hardness:
- not reported
- Test temperature:
- 21.2 °C
- pH:
- not reported
- Dissolved oxygen:
- endpoint of the study: change of oxygen concentration
- Salinity:
- not applicable
- Nominal and measured concentrations:
- main test 1: nominal 300 and 10000 mg/L
main test 2: nominal 10, 30 and 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer 100 mL
- Type: not reported
- Material, size, headspace, fill volume: glass. 100 mL, 100 mL
- Aeration: direct
- No. of organisms per vessel: 1-3 mL bacteria suspension
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to DIN 38412, part 27, point 8.1
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
- Culture medium different from test medium: not reported
- Intervals of water quality measurement: not applicable
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not reported
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): change in oxygen concentration
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
main test 1: 33.3
main test 2: 3-3.3 - Reference substance (positive control):
- no
- Key result
- Duration:
- 3.8 min
- Dose descriptor:
- EC0
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: decrease in oxygen consumption
- Key result
- Duration:
- 3.8 min
- Dose descriptor:
- EC10
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: decrease of oxygen consumption
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a valid and reliable study according to DIN 38412, part 27 (1992), the inhibition of oxygen consumption of Pseudomonas putida was measured. The EC0 and EC10 were 30 and 100 mg/L, respectively.
- Executive summary:
The acute toxicity of the test substance Sandelice to bacteria was determined using the test for inhibition of the oxygen consumption according to DIN 38412, part 27 (1992). The tested concentration range was 10 -10000 mg/L. The following effect concentrations were determined:
EC0: 30 mg/L
EC10: 100 mg/L
Reference
Table 1 Oxygen consumption in main test 1
Blind value | test concentration (mg/L) | replicates | measurement (mg O2 per L and per min |
1 | --- | --- | 2.09 |
2 | --- | --- | 2.07 |
--- | 10000 | 1 | 1.58 |
--- | 10000 | 2 | 1.66 |
--- | 300 | 1 | 1.51 |
--- | 300 | 2 | 1.48 |
3 | --- | --- | 1.75 |
4 | --- | --- | 1.87 |
Table 2 Oxygen consumption in main test 2
Blind value | test concentration (mg/L) | replicates | measurement (mg O2 per L and per min |
5 | --- | --- | 1.93 |
6 | --- | --- | 2.21 |
--- | 30 | 1 | 1.82 |
--- | 30 | 2 | 1.99 |
--- | 10 | 1 | 1.98 |
--- | 10 | 2 | 2.21 |
--- | 100 | 1 | 1.80 |
--- | 100 | 2 | 1.92 |
7 | --- | --- | 1.96 |
8 | --- | --- | 2.15 |
Table 3 Oxygen consumption relative to the blind values
Test concentration (mg/L) | Mean of inhibition (+) or advancement (-) from two replicates (%) |
10 | -1.5 |
30 | +7.6 |
100 | +9.8 |
300 | +23.1 |
10000 | +16.8 |
Description of key information
The acute toxicity of the test substance to bacteria was determined using the test for inhibition of the oxygen consumption according to DIN 38412, part 27 (1992). The tested concentration range was 10 -10000 mg/L.
The following effect concentrations were determined:EC0: 30 mg/L
EC10: 100 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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