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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-piperidin-2-ylethanol
EC Number:
216-059-2
EC Name:
2-piperidin-2-ylethanol
Cas Number:
1484-84-0
Molecular formula:
C7H15NO
IUPAC Name:
2-piperidin-2-ylethanol
Constituent 2
Reference substance name:
2-Piperidineethanol
IUPAC Name:
2-Piperidineethanol

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Pseudokirchneriella subcapitata HINDÁK, SAG 61.81;

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Hardness:
0.24 mmol Ca+Mg/L.

Test temperature:
Temperature (target) Nominal range: 21 - 24°C, controlled at ± 2°C
pH:
8.1 +- 0.2
Nominal and measured concentrations:
5 concentrations were tested in a geometrical series with a
dilution factor of 2: 6.25 - 12.5 - 25.0 - 50.0 - 100 mg/L.
Details on test conditions:
Biological Parameters
The cell density was measured daily via Chlorophyll-a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal.
Microscopic evaluation of the cells at the start and the end of the incubation period was carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algae cells.

Results and discussion

Effect concentrations
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

The measured concentrations of the TS in the fresh media (0 h) were between 105 and 110 % of the nominal values. The measured concentrations in the old media (72 h) containing algae were in the range of 106 to 115 % of the nominal values.All effect values given are based on the nominal test item concentrations of 2-Piperidinethanol. For results, please refer toTable1.

 

Growth Rate Inhibition
Nominal test item concentration [mg/L]

NOEC

6.25

LOEC

12.5

ErC10

24.9 (21.4 – 28.5)

ErC20

37.3 (34.5 – 39.6)

ErC50

63.7 (60.3 – 67.8)

 

Yield Inhibition

Nominal test item concentration [mg/L]

NOEC

6.25

LOEC

12.5

EyC10

7.52 (< 6.25 – 11.6)

EyC20

13.5 (10.2 – 17.2)

EyC50

27.6 (23.9 – 31.7)

Applicant's summary and conclusion

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