2-piperidin-2-ylethanol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-piperidin-2-ylethanol

EC Number:

216-059-2

EC Name:

2-piperidin-2-ylethanol

Cas Number:

1484-84-0

Molecular formula:

C7H15NO

IUPAC Name:

2-piperidin-2-ylethanol

Test material form:

not specified

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Inoculum of the aqueous phase of non-adapted activated sludge.

Duration of test (contact time):

28 d

Details on study design:

The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air until test start. 10 mL/L of this mixture were used to initiate inoculation.

Results and discussion

% Degradation

Sampling time:

28 d

Remarks on result:

readily biodegradable based on QSAR/QSPR prediction

Details on results:

The adaptation phase of the functional control changed within 4 days into the degradation phase (degradation 10 %). The course of the degradation was rapid and the functional control reached the pass level of 60 % within 11 days and a maximum biodegradation of 82 % on day 28. The validity criterion degradation k 60 % after 14 days is fulfilled.In the toxicity control containing both test and reference item a biodegradation of 58 % was determined within 14 days and it came to 89 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The mean of test item replicates reached the 10 % level (beginning of biodegradation) within 8 days. The 60 % pass level was reached by both test item replicates within 25 days. The mean biodegradation after 28 days was 97 %.
Under the test conditions the test item is classified as readily biodegradable within the 28 day period of the study, but without complying to the 10-day-window.In the inoculum control the total CO2 production was 26.8 mg CO2/L after 28 days.

Any other information on results incl. tables

Table 2:CO2-Production and Biodegradation after 28 Days

 

CO2-Production	Functional Control	Test 1	Item 2	Toxicity Control test item + reference item
Net      [mg/3 L]	104.2	110.2	112.8	215.4
[mg/L]	34.7	36.7	37.6	71.8
Theor.  [mg/3 L]	127.8	114.7		242.5
[mg/L]	42.6	38.2		80.8
Degradation [%]	82	96	98	89

 

Table 3:CO2-Production and Biodegradation in the Inoculum Control, the Functional Control and the Toxicity Control

Study Day	Date	Inoculum Control [mg CO2/3 L] mv	[mg CO2/3 Gross	Functional L] Net Sum	Control Degr. [%]	[mg CO2/3 Gross	Toxicity Control L] Net Sum	Degr. [%]
1	2016-04-01	4.1	4.8	0.7	1	12.7	8.6	4
4	2016-04-04	12.2	36.4	24.9	19	53.9	50.3	21
6	2016-04-06	8.1	35.1	51.9	41	41.4	83.6	34
8	2016-04-08	6.6	23.1	68.4	54	22.8	99.8	41
11	2016-04-11	7.6	20.6	81.4	64	21.9	114.1	47
14	2016-04-14	7.7	14.6	88.3	69	34.7	141.1	58
18	2016-04-18	7.5	12.8	93.6	73	27.6	161.2	66
21	2016-04-22	9.8	13.4	97.2	76	32.1	183.5	76
25	2016-04-25	5.6	7.9	99.5	78	25.5	203.4	84
28	2016-04-28	6.9	8.6	101.2	79	15.7	212.2	88
29*	2016-04-29	4.3	7.3	104.2	82	7.5	215.4	89

Degr. = degradation            mv = mean value            *) results of last two gas wash bottles

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Under the test conditions the test item is classified as
readily biodegradable
within the 28 day period of the study, but without complying to the 10-day-window.