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Long-term toxicity to fish

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Description of key information

The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC (lowest observed effect concentration) and the NOEC (No observed effect concentration) of the test substance to juvenile Rare minnow were concluded as follows:
28 d-LOEC > 100 mg/L (measured concentration 96.9 mg/L);
28 d-NOEC = 100 mg/L (measured concentration 96.9 mg/L).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Under semi-static conditions of 72 h-renewal, the effect on growth rate sand other observed effects in juvenile fish (Gobiocypris rarus) exposed to thetest substance for 28 days was conducted according to the following guidelines: “The guidelines for the testing of chemicals”,SEPA(HJ/T 153-2004);Procedure 215of theGuidelines for Testing of Chemicalsof the OECD: " Fish, Juvenile Growth Test” (2000)etc.

During the whole test period, the pH values of the control and Test Media were between 5.12 and 7.47, and the Dissolved Oxygen (DO) values varied from 85% to 92% of the air saturation at the test temperature, and the temperature of the Test Media was maintained in 23.0°C~5°C. All fishes in the control group were normal. The mean weight of fish in the controls increased more than 50%. So the study met the acceptability criteria prescribed by the protocol (dissolved oxygen concentration: no less than 60% of the air saturation value; temperature: (23±2)oC and not differ by more than ±1oC between test chambers; the increasing rate of the mean wet-weight of fish: no less than 50% of the initial weight). Therefore the test was considered valid.

The concentration ofthe test item in test medium was determined by ICP-MS to represent the stability of the test substance in test period. A linear regression equation was obtained with the ICP-MS response values of phosphorus (y) vs. the concentration of the test item (mg/L): A=1172.4c+237.03, with good linearity of R=0.989. The results show that linearity for the concentration range of 0.02 mg/L to 0.50 mg/L is good. Besides, the minimum detection amount of ICP-MS for the test item is 1.0×10-10g, and the minimum detection concentration for water sample is 0.01mg/L. The analytical results showed that the concentration of the test item is consistent in the test medium throughout a period of 72 h (deviation within 20%). Thus a semi-static test of 72 h-renewal was reasonable.

The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC (lowest observed effect concentration) and the NOEC (No observed effect concentration) of the test substance to juvenile Rare minnow were concluded as follows:

28 d-LOEC > 100 mg/L(measured concentration 96.9 mg/L);

28 d-NOEC = 100 mg/L(measured concentration 96.9 mg/L).

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