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EC number: 700-733-2 | CAS number: -
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC (lowest observed effect concentration) and the NOEC (No observed effect concentration) of the test substance to juvenile Rare minnow were concluded as follows:
28 d-LOEC > 100 mg/L (measured concentration 96.9 mg/L);
28 d-NOEC = 100 mg/L (measured concentration 96.9 mg/L).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Under semi-static conditions of 72 h-renewal, the effect on growth rate sand other observed effects in juvenile fish (Gobiocypris rarus) exposed to thetest substance for 28 days was conducted according to the following guidelines: “The guidelines for the testing of chemicals”,SEPA(HJ/T 153-2004);Procedure 215of the“Guidelines for Testing of Chemicals”of the OECD: " Fish, Juvenile Growth Test” (2000)etc.
During the whole test period, the pH values of the control and Test Media were between 5.12 and 7.47, and the Dissolved Oxygen (DO) values varied from 85% to 92% of the air saturation at the test temperature, and the temperature of the Test Media was maintained in 23.0°C~5°C. All fishes in the control group were normal. The mean weight of fish in the controls increased more than 50%. So the study met the acceptability criteria prescribed by the protocol (dissolved oxygen concentration: no less than 60% of the air saturation value; temperature: (23±2)oC and not differ by more than ±1oC between test chambers; the increasing rate of the mean wet-weight of fish: no less than 50% of the initial weight). Therefore the test was considered valid.
The concentration ofthe test item in test medium was determined by ICP-MS to represent the stability of the test substance in test period. A linear regression equation was obtained with the ICP-MS response values of phosphorus (y) vs. the concentration of the test item (mg/L): A=1172.4c+237.03, with good linearity of R=0.989. The results show that linearity for the concentration range of 0.02 mg/L to 0.50 mg/L is good. Besides, the minimum detection amount of ICP-MS for the test item is 1.0×10-10g, and the minimum detection concentration for water sample is 0.01mg/L. The analytical results showed that the concentration of the test item is consistent in the test medium throughout a period of 72 h (deviation within 20%). Thus a semi-static test of 72 h-renewal was reasonable.
The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC (lowest observed effect concentration) and the NOEC (No observed effect concentration) of the test substance to juvenile Rare minnow were concluded as follows:
28 d-LOEC > 100 mg/L(measured concentration 96.9 mg/L);
28 d-NOEC = 100 mg/L(measured concentration 96.9 mg/L).Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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