Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2007
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Details on sampling:
- Concentrations: 1000 mg/l

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
18-22°C
pH:
6.95
Dissolved oxygen:
permanent aeration
Nominal and measured concentrations:
nominal: 1000 mg/l
Reference substance (positive control):
yes
Remarks:
Copper (II) sulphate pentahydrate

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Reported statistics and error estimates:
The two control respiration rates differ not more than 15% in the study. The EC50 (3 h) of copper(II) sulphate pentahydrate is in the accepted range of 53-157 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
the test item is not toxic at concentrations