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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 29,4 mg/l; limit concentration: saturated solution (100 mg/l)

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the stock solution (100mg/l test item) was shaken with 20rpm for 24 h at ambient temperature. After 24 h undissolved particles were removed by centrifugation for 20 min at 3000 rpm.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Straus (Clone 5)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
no

Test conditions

Hardness:
160 - 180 mg CaCO3/l
Test temperature:
19-20°C
pH:
7.72-7.95
Dissolved oxygen:
6.50-8.14 mg/l
Nominal and measured concentrations:
nominal: 100 mg/l
mean measured: 29.4 mg/l
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 29.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
> 29.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
> 29.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: no
- Mortality of control: no
Results with reference substance (positive control):
- Results with reference substance valid? y
- EC50: 1.62 mg/l (CI 1.59-1.65 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No effects on Daphnia Magna until the limit of water solubility of 29.4 mg/l;